Synthetic cationic amphiphiles for liposome-mediated DNA transfection

A. Ito, R. Miyazoe, J. Y. Mitoma, T. Akao, T. Osaki, T. Kunitake

研究成果: Contribution to journalArticle査読

51 被引用数 (Scopus)

抄録

The compounds with efficient DNA transfection ability into eukaryotic cells were searched from various synthetic amphiphiles which have cationic heads and long saturated hydrocarbon tails. The efficiency of amphiphiles in gene transfer was examined by the transient expression of cytochrome b5 from its cDNA in COS cells. Among various synthetic amphiphiles, including N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethyl-ammonium chloride which is commercially available lipid, O,O'-didodecyl-N-[p-(2-trimethylammonioethyloxy)benzoyl]-(L)-glutamate bromide was highest in efficiency. The optimum condition for the amount of the amphiphile and DNA, and the incubation time were established to be 7.5-15 μg/22 mm dish and 1-10 μg/22 mm dish, and 48-72 h, respectively.

本文言語英語
ページ(範囲)235-241
ページ数7
ジャーナルBiochemistry International
22
2
出版ステータス出版済み - 12 1 1990

All Science Journal Classification (ASJC) codes

  • 生化学

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