Synthetic microbial consortium with specific roles designated by genetic circuits for cooperative chemical production

Hiroshi Honjo, Kenshiro Iwasaki, Yuki Soma, K. Tsuruno, Hiroyuki Hamada, T. Hanai

研究成果: Contribution to journalArticle査読

21 被引用数 (Scopus)

抄録

Synthetic microbial consortia consisting of microorganisms with different synthetic genetic circuits or divided synthetic metabolic pathway components can exert functions that are beyond the capacities of single microorganisms. However, few consortia of microorganisms with different synthetic genetic circuits have been developed. We designed and constructed a synthetic microbial consortium composed of an enzyme-producing strain and a target chemical-producing strain using Escherichia coli for chemical production with efficient saccharification. The enzyme-producing strain harbored a synthetic genetic circuit to produce beta-glucosidase, which converts cellobiose to glucose, destroys itself via the lytic genes, and release the enzyme when the desired cell density is reached. The target chemical-producing strain was programmed by a synthetic genetic circuit to express enzymes in the synthetic metabolic pathway for isopropanol production when the enzyme-producing strain grows until release of the enzyme. Our results demonstrate the benefits of synthetic microbial consortia with distributed tasks for effective chemical production from biomass.

本文言語英語
ページ(範囲)268-275
ページ数8
ジャーナルMetabolic Engineering
55
DOI
出版ステータス出版済み - 9 2019

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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