抄録
Synthetic microbial consortia consisting of microorganisms with different synthetic genetic circuits or divided synthetic metabolic pathway components can exert functions that are beyond the capacities of single microorganisms. However, few consortia of microorganisms with different synthetic genetic circuits have been developed. We designed and constructed a synthetic microbial consortium composed of an enzyme-producing strain and a target chemical-producing strain using Escherichia coli for chemical production with efficient saccharification. The enzyme-producing strain harbored a synthetic genetic circuit to produce beta-glucosidase, which converts cellobiose to glucose, destroys itself via the lytic genes, and release the enzyme when the desired cell density is reached. The target chemical-producing strain was programmed by a synthetic genetic circuit to express enzymes in the synthetic metabolic pathway for isopropanol production when the enzyme-producing strain grows until release of the enzyme. Our results demonstrate the benefits of synthetic microbial consortia with distributed tasks for effective chemical production from biomass.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 268-275 |
| ページ数 | 8 |
| ジャーナル | Metabolic Engineering |
| 巻 | 55 |
| DOI | |
| 出版物ステータス | 出版済み - 9 1 2019 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Bioengineering
- Applied Microbiology and Biotechnology
これを引用
Synthetic microbial consortium with specific roles designated by genetic circuits for cooperative chemical production. / Honjo, Hiroshi; Iwasaki, Kenshiro; Soma, Yuki; Tsuruno, K.; Hamada, Hiroyuki; Hanai, Taizo.
：: Metabolic Engineering, 巻 55, 01.09.2019, p. 268-275.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - Synthetic microbial consortium with specific roles designated by genetic circuits for cooperative chemical production
AU - Honjo, Hiroshi
AU - Iwasaki, Kenshiro
AU - Soma, Yuki
AU - Tsuruno, K.
AU - Hamada, Hiroyuki
AU - Hanai, Taizo
PY - 2019/9/1
Y1 - 2019/9/1
N2 - Synthetic microbial consortia consisting of microorganisms with different synthetic genetic circuits or divided synthetic metabolic pathway components can exert functions that are beyond the capacities of single microorganisms. However, few consortia of microorganisms with different synthetic genetic circuits have been developed. We designed and constructed a synthetic microbial consortium composed of an enzyme-producing strain and a target chemical-producing strain using Escherichia coli for chemical production with efficient saccharification. The enzyme-producing strain harbored a synthetic genetic circuit to produce beta-glucosidase, which converts cellobiose to glucose, destroys itself via the lytic genes, and release the enzyme when the desired cell density is reached. The target chemical-producing strain was programmed by a synthetic genetic circuit to express enzymes in the synthetic metabolic pathway for isopropanol production when the enzyme-producing strain grows until release of the enzyme. Our results demonstrate the benefits of synthetic microbial consortia with distributed tasks for effective chemical production from biomass.
AB - Synthetic microbial consortia consisting of microorganisms with different synthetic genetic circuits or divided synthetic metabolic pathway components can exert functions that are beyond the capacities of single microorganisms. However, few consortia of microorganisms with different synthetic genetic circuits have been developed. We designed and constructed a synthetic microbial consortium composed of an enzyme-producing strain and a target chemical-producing strain using Escherichia coli for chemical production with efficient saccharification. The enzyme-producing strain harbored a synthetic genetic circuit to produce beta-glucosidase, which converts cellobiose to glucose, destroys itself via the lytic genes, and release the enzyme when the desired cell density is reached. The target chemical-producing strain was programmed by a synthetic genetic circuit to express enzymes in the synthetic metabolic pathway for isopropanol production when the enzyme-producing strain grows until release of the enzyme. Our results demonstrate the benefits of synthetic microbial consortia with distributed tasks for effective chemical production from biomass.
UR - http://www.scopus.com/inward/record.url?scp=85070527411&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85070527411&partnerID=8YFLogxK
U2 - 10.1016/j.ymben.2019.08.007
DO - 10.1016/j.ymben.2019.08.007
M3 - Article
VL - 55
SP - 268
EP - 275
JO - Metabolic Engineering
JF - Metabolic Engineering
SN - 1096-7176
ER -