TY - JOUR
T1 - Synthetic-Molecule/Protein Hybrid Probe with Fluorogenic Switch for Live-Cell Imaging of DNA Methylation
AU - Hori, Yuichiro
AU - Otomura, Norimichi
AU - Nishida, Ayuko
AU - Nishiura, Miyako
AU - Umeno, Maho
AU - Suetake, Isao
AU - Kikuchi, Kazuya
N1 - Funding Information:
This research was supported by JSPS KAKENHI (Grant Nos. JP25220207, JP16H00768, JP15K12754, and JP16K13099 to K.K. and JP26282215, JP17H02210, JP16K13088, JP17H06005, and JP16H01428 “Resonance Bio” to Y.H.), by AMED-CREST, and by SICORP from JST.
Funding Information:
This research was supported by JSPS KAKENHI (Grant Nos. JP25220207, JP16H00768, JP15K12754, and JP16K13099 to K.K. and JP26282215, JP17H02210, JP16K13088, JP17H06005, and JP16H01428 Resonance Bio to Y.H.), by AMED-CREST, and by SICORP from JST.
Publisher Copyright:
© 2018 American Chemical Society.
PY - 2018/2/7
Y1 - 2018/2/7
N2 - Hybrid probes consisting of synthetic molecules and proteins are powerful tools for detecting biological molecules and signals in living cells. To date, most targets of the hybrid probes have been limited to pH and small analytes. Although biomacromolecules are essential to the physiological function of cells, the hybrid-probe-based approach has been scarcely employed for live-cell detection of biomacromolecules. Here, we developed a hybrid probe with a chemical switch for live-cell imaging of methylated DNA, an important macromolecule in the repression of gene expression. Using a protein labeling technique, we created a hybrid probe containing a DNA-binding fluorogen and a methylated-DNA-binding domain. The hybrid probe enhanced fluorescence intensity upon binding to methylated DNA and successfully monitored methylated DNA during mitosis. The hybrid probe offers notable advantages absent from probes based on small molecules or fluorescent proteins and is useful for live-cell analyses of epigenetic phenomena and diseases related to DNA methylation.
AB - Hybrid probes consisting of synthetic molecules and proteins are powerful tools for detecting biological molecules and signals in living cells. To date, most targets of the hybrid probes have been limited to pH and small analytes. Although biomacromolecules are essential to the physiological function of cells, the hybrid-probe-based approach has been scarcely employed for live-cell detection of biomacromolecules. Here, we developed a hybrid probe with a chemical switch for live-cell imaging of methylated DNA, an important macromolecule in the repression of gene expression. Using a protein labeling technique, we created a hybrid probe containing a DNA-binding fluorogen and a methylated-DNA-binding domain. The hybrid probe enhanced fluorescence intensity upon binding to methylated DNA and successfully monitored methylated DNA during mitosis. The hybrid probe offers notable advantages absent from probes based on small molecules or fluorescent proteins and is useful for live-cell analyses of epigenetic phenomena and diseases related to DNA methylation.
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U2 - 10.1021/jacs.7b09713
DO - 10.1021/jacs.7b09713
M3 - Article
C2 - 29381073
AN - SCOPUS:85041892199
SN - 0002-7863
VL - 140
SP - 1686
EP - 1690
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 5
ER -