Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide

Zhiyang Zeng, Yu Hoshino, Andy Rodriguez, Hoseong Yoo, Kenneth J. Shea

研究成果: Contribution to journalArticle査読

94 被引用数 (Scopus)

抄録

Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide have been prepared by inverse microemulsion polymerization. Peptide affinity was achieved in part by incorporating the target (imprint) peptide in the polymerization reaction mixture. Incorporation of the imprint peptide assists in the creation of complementary binding sites in the resulting polymer nanoparticle (NP). To orient the imprint peptide at the interface of the water and oil domains during polymerization, the peptide target was coupled with fatty acid chains of varying length. The peptide-NP binding affinities (ca. 90-900 nM) were quantitatively evaluated by a quartz crystal microbalance (QCM). The optimal chain length was established that created high affinity peptide binding sites on the surface of the nanoparticles. This method can be used for the preparation of nanosized synthetic polymers with antibody-like affinity for hydrophilic peptides and proteins ("plastic antibodies").

本文言語英語
ページ(範囲)199-204
ページ数6
ジャーナルACS nano
4
1
DOI
出版ステータス出版済み - 1 26 2010
外部発表はい

All Science Journal Classification (ASJC) codes

  • 材料科学(全般)
  • 工学(全般)
  • 物理学および天文学(全般)

フィンガープリント

「Synthetic polymer nanoparticles with antibody-like affinity for a hydrophilic peptide」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル