Systemic priming of alloreactive cytotoxic cells in carp, following anal administration of allogeneic cell antigens

Atsushi Sato, Tomonori Somamoto, Hiroyuki Yokooka, Nobuaki Okamoto

研究成果: ジャーナルへの寄稿学術誌査読

22 被引用数 (Scopus)

抄録

In the present study, we confirmed that cellular immune responses, especially specific cell-mediated cytotoxicity, could be induced in systemic carp leucocytes, following anal administration of antigens. Effector cells isolated from systemic lymphoid tissues (head kidney, spleen and peripheral blood) of carp that were immunised anally with allogeneic cells (EPC or KG cell line) efficiently lysed immunogenic target cells. The lytic activity was increased as a result of secondary sensitisation and peaked around 7 days after the final immunisation. In some aspects, the alloantigen-specific cell-mediated cytotoxicity induced by anal sensitisation was different from that induced by intraperitoneal (i.p.) injection. First, the activity induced by anal immunisation was higher than that resulting from i.p. immunisation when fish were immunised twice with a 7-day interval, whereas similar kinetics of the cytotoxicity were observed after the final immunisation. Second, repeated anal administrations tended to decrease the cytotoxic activity, although repeated i.p. injections increased the activity. These findings indicate that the anal administration of antigens in fish can elicit and modulate cellular immune responses.

本文言語英語
ページ(範囲)43-52
ページ数10
ジャーナルFish and Shellfish Immunology
19
1
DOI
出版ステータス出版済み - 7月 2005
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 環境化学
  • 水圏科学

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