T-cell receptor repertoires of tumor-infiltrating lymphocytes after hyperthermia using functionalized magnetite nanoparticles

Akira Ito, Masaki Yamaguchi, Noriaki Okamoto, Yuji Sanematsu, Yoshinori Kawabe, Kazumasa Wakamatsu, Shosuke Ito, Hiroyuki Honda, Takeshi Kobayashi, Eiichi Nakayama, Yasuaki Tamura, Masae Okura, Toshiharu Yamashita, Kowichi Jimbow, Masamichi Kamihira

研究成果: ジャーナルへの寄稿学術誌査読

18 被引用数 (Scopus)

抄録

Aim: Accumulating evidence has indicated that hyperthermia using magnetite nanoparticles induces antitumor immunity. This study investigated the diversity of T-cell receptors (TCRs) in tumor-infiltrating lymphocytes after hyperthermia using magnetite nanoparticles.Materials & methods: Functionalized magnetite nanoparticles, N-propionyl-4-S-cysteaminylphenol (NPrCAP)/magnetite, were synthesized by conjugating the melanogenesis substrate NPrCAP with magnetite nanoparticles. NPrCAP/magnetite nanoparticles were injected into B16 melanomas in C57BL/6 mice, which were subjected to an alternating magnetic field for hyperthermia treatment. Results: Enlargement of the tumor-draining lymph nodes was observed after hyperthermia. The TCR repertoire was restricted in tumor-infiltrating lymphocytes, and expansion of Vβ11+ T cells was preferentially found. DNA sequences of the third complementaritydetermining regions revealed the presence of clonally expanded T cells. Conclusion: These results indicate that the T-cell response in B16 melanomas after hyperthermia is dominated by T cells directed toward a limited number of epitopes and that epitope-specific T cells frequently use a restricted TCR repertoire.

本文言語英語
ページ(範囲)891-902
ページ数12
ジャーナルNanomedicine
8
6
DOI
出版ステータス出版済み - 6月 2013

!!!All Science Journal Classification (ASJC) codes

  • バイオエンジニアリング
  • 医学(その他)
  • 生体医工学
  • 材料科学(全般)

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