Tannic acid enhancing insecticidal activity of protoxin produced in Bacillus thuringiensis subsp. Kurstaki KB100 Strain Against Spodoptera exigua

Na Young Jin, You Kyoung Lee, Bo Ram Lee, Yu Seop Kim, Jun Hack Jun, Mi Ja Seo, Young Nam Youn, Chisa Yasunaga-Aoki, Yong Man Yu

研究成果: ジャーナルへの寄稿記事

3 引用 (Scopus)

抄録

Insecticidal activity was enhanced when Bacillus thuringiensis subsp. kurstaki KB100 strain containing insecticidal activity against Spodoptera exigua was mixed with tannic acid, a protease inhibitor. To investigate the cause of this result, inhibition rate of tannic acid against protease activity in midgut juice of S. exigua was measured. As a result, it was found that protease activity in midgut juice of 5. exigua was about 83.1%, 77.6%, 68.0%, and 40.1% at concentrations of 10, 20, 40 and 80 mM, respectively. Such reduction of protease activity was measured with increasing concentration. Analysis of substrate reaction in several kinds of proteases was performed. Trypsin exhibited 91.4% and 89.4% of proteolytic activity in BApNA and BPVApNA substrates, respectively. Thus, when Trypsin was treated with tannic acid, proteolytic activity was 62.2% and 54.5% in BApNA and BPVApNA substrates, respectively. Trypsin's proteolytic activity was inhibited by 29.2% and 34.9% when mixed with tannic acid. However, proteolytic activity of chymotrypsin and elastase was not inhibited by tannic acid. Digestion of protoxin produced in B. thuringiensis KB100 strain by Trypsin was analyzed using SDS-PAGE. As a result, a band appeared at about 60 kDa-70 kDa and it seemed to be inhibited by tannic acid. Digestion patterns of protoxin were measured over time. As it was over-digested in a group of Trypsin treatment, a band of protoxin at 60 kDa completely disappeared. On the other hand, when it was treated with tannic acid, a digestion inhibitor, protoxin was maintained for up to 24 hours.

元の言語英語
ページ(範囲)97-102
ページ数6
ジャーナルJournal of the Faculty of Agriculture, Kyushu University
60
発行部数1
出版物ステータス出版済み - 2 1 2015

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Bacillus thuringiensis subsp. kurstaki
Spodoptera
Bacillus thuringiensis
Spodoptera exigua
Tannins
tannins
Trypsin
trypsin
Peptide Hydrolases
proteinases
Digestion
digestion
midgut
juices
elastase
Pancreatic Elastase
Chymotrypsin
chymotrypsin
proteinase inhibitors
Protease Inhibitors

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

これを引用

Tannic acid enhancing insecticidal activity of protoxin produced in Bacillus thuringiensis subsp. Kurstaki KB100 Strain Against Spodoptera exigua. / Jin, Na Young; Lee, You Kyoung; Lee, Bo Ram; Kim, Yu Seop; Jun, Jun Hack; Seo, Mi Ja; Youn, Young Nam; Yasunaga-Aoki, Chisa; Yu, Yong Man.

:: Journal of the Faculty of Agriculture, Kyushu University, 巻 60, 番号 1, 01.02.2015, p. 97-102.

研究成果: ジャーナルへの寄稿記事

Jin, Na Young ; Lee, You Kyoung ; Lee, Bo Ram ; Kim, Yu Seop ; Jun, Jun Hack ; Seo, Mi Ja ; Youn, Young Nam ; Yasunaga-Aoki, Chisa ; Yu, Yong Man. / Tannic acid enhancing insecticidal activity of protoxin produced in Bacillus thuringiensis subsp. Kurstaki KB100 Strain Against Spodoptera exigua. :: Journal of the Faculty of Agriculture, Kyushu University. 2015 ; 巻 60, 番号 1. pp. 97-102.
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title = "Tannic acid enhancing insecticidal activity of protoxin produced in Bacillus thuringiensis subsp. Kurstaki KB100 Strain Against Spodoptera exigua",
abstract = "Insecticidal activity was enhanced when Bacillus thuringiensis subsp. kurstaki KB100 strain containing insecticidal activity against Spodoptera exigua was mixed with tannic acid, a protease inhibitor. To investigate the cause of this result, inhibition rate of tannic acid against protease activity in midgut juice of S. exigua was measured. As a result, it was found that protease activity in midgut juice of 5. exigua was about 83.1{\%}, 77.6{\%}, 68.0{\%}, and 40.1{\%} at concentrations of 10, 20, 40 and 80 mM, respectively. Such reduction of protease activity was measured with increasing concentration. Analysis of substrate reaction in several kinds of proteases was performed. Trypsin exhibited 91.4{\%} and 89.4{\%} of proteolytic activity in BApNA and BPVApNA substrates, respectively. Thus, when Trypsin was treated with tannic acid, proteolytic activity was 62.2{\%} and 54.5{\%} in BApNA and BPVApNA substrates, respectively. Trypsin's proteolytic activity was inhibited by 29.2{\%} and 34.9{\%} when mixed with tannic acid. However, proteolytic activity of chymotrypsin and elastase was not inhibited by tannic acid. Digestion of protoxin produced in B. thuringiensis KB100 strain by Trypsin was analyzed using SDS-PAGE. As a result, a band appeared at about 60 kDa-70 kDa and it seemed to be inhibited by tannic acid. Digestion patterns of protoxin were measured over time. As it was over-digested in a group of Trypsin treatment, a band of protoxin at 60 kDa completely disappeared. On the other hand, when it was treated with tannic acid, a digestion inhibitor, protoxin was maintained for up to 24 hours.",
author = "Jin, {Na Young} and Lee, {You Kyoung} and Lee, {Bo Ram} and Kim, {Yu Seop} and Jun, {Jun Hack} and Seo, {Mi Ja} and Youn, {Young Nam} and Chisa Yasunaga-Aoki and Yu, {Yong Man}",
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T1 - Tannic acid enhancing insecticidal activity of protoxin produced in Bacillus thuringiensis subsp. Kurstaki KB100 Strain Against Spodoptera exigua

AU - Jin, Na Young

AU - Lee, You Kyoung

AU - Lee, Bo Ram

AU - Kim, Yu Seop

AU - Jun, Jun Hack

AU - Seo, Mi Ja

AU - Youn, Young Nam

AU - Yasunaga-Aoki, Chisa

AU - Yu, Yong Man

PY - 2015/2/1

Y1 - 2015/2/1

N2 - Insecticidal activity was enhanced when Bacillus thuringiensis subsp. kurstaki KB100 strain containing insecticidal activity against Spodoptera exigua was mixed with tannic acid, a protease inhibitor. To investigate the cause of this result, inhibition rate of tannic acid against protease activity in midgut juice of S. exigua was measured. As a result, it was found that protease activity in midgut juice of 5. exigua was about 83.1%, 77.6%, 68.0%, and 40.1% at concentrations of 10, 20, 40 and 80 mM, respectively. Such reduction of protease activity was measured with increasing concentration. Analysis of substrate reaction in several kinds of proteases was performed. Trypsin exhibited 91.4% and 89.4% of proteolytic activity in BApNA and BPVApNA substrates, respectively. Thus, when Trypsin was treated with tannic acid, proteolytic activity was 62.2% and 54.5% in BApNA and BPVApNA substrates, respectively. Trypsin's proteolytic activity was inhibited by 29.2% and 34.9% when mixed with tannic acid. However, proteolytic activity of chymotrypsin and elastase was not inhibited by tannic acid. Digestion of protoxin produced in B. thuringiensis KB100 strain by Trypsin was analyzed using SDS-PAGE. As a result, a band appeared at about 60 kDa-70 kDa and it seemed to be inhibited by tannic acid. Digestion patterns of protoxin were measured over time. As it was over-digested in a group of Trypsin treatment, a band of protoxin at 60 kDa completely disappeared. On the other hand, when it was treated with tannic acid, a digestion inhibitor, protoxin was maintained for up to 24 hours.

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