Targets and dynamics of promoter DNA methylation during early mouse development

Julie Borgel, Sylvain Guibert, Yufeng Li, Hatsune Chiba, Dirk Schübeler, Hiroyuki Sasaki, Thierry Forné, Michael Weber

    研究成果: Contribution to journalArticle査読

    430 被引用数 (Scopus)

    抄録

    DNA methylation is extensively reprogrammed during the early phases of mammalian development, yet genomic targets of this process are largely unknown. We optimized methylated DNA immunoprecipitation for low numbers of cells and profiled DNA methylation during early development of the mouse embryonic lineage in vivo. We observed a major epigenetic switch during implantation at the transition from the blastocyst to the postimplantation epiblast. During this period, DNA methylation is primarily targeted to repress the germline expression program. DNA methylation in the epiblast is also targeted to promoters of lineage-specific genes such as hematopoietic genes, which are subsequently demethylated during terminal differentiation. De novo methylation during early embryogenesis is catalyzed by Dnmt3b, and absence of DNA methylation leads to ectopic gene activation in the embryo. Finally, we identify nonimprinted genes that inherit promoter DNA methylation from parental gametes, suggesting that escape of post-fertilization DNA methylation reprogramming is prevalent in the mouse genome.

    本文言語英語
    ページ(範囲)1093-1100
    ページ数8
    ジャーナルNature genetics
    42
    12
    DOI
    出版ステータス出版済み - 12 2010

    All Science Journal Classification (ASJC) codes

    • 遺伝学

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