TCDD disrupts posterior palatogenesis and causes cleft palate

Tomohiro Yamada, Azumi Hirata, Eri Sasabe, Tomohide Yoshimura, Seiji Ohno, Naoya Kitamura, Tetsuya Yamamoto

研究成果: Contribution to journalArticle査読

24 被引用数 (Scopus)

抄録

Dioxins (e.g. 2,3,7,8-tetrachlorodibenzo-p-dioxin; TCDD) cause cleft palate at a high rate. A post-fusional split may contribute to the pathogenesis, and tissue fragility may be a concern. The objective of this study was to investigate the effects of TCDD on the palatal epithelium, bone and muscle, which contribute to tissue integrity. ICR mice (10-12 weeks old) were used. TCDD was administered on E12.5 at 40 mg/kg. Immunohistochemical staining for AhR, ER-α, laminin, collagen IV, osteopontin, Runx2, MyoD, and desmin were performed. Furthermore, western blot analysis for osteopontin, Runx2, MyoD, and desmin were performed to evaluate protein expression in the palatal tissue. Immunohistologically, there was little difference in the collagen IV and laminin localization in the palatal epithelium between control versus TCDD-treated mice. Runx2 and osteopontin immunoreactivity decreased in the TCDD-treated palatal bone, and MyoD and desmin decreased in the TCDD-treated palatal muscle. AhR and ER-α immunoreactivity were localized to the normal palatal bone, but ER-α was diminished in the TCDD-treated palate. On western blot analysis, Runx2, MyoD, and desmin were all downregulated in the TCDD-treated palate. TCDD may suppress palatal osteogenesis and myogenesis via AhR, and cause cleft palates via a post-fusional split mechanism, in addition to a failure of palatal fusion.

本文言語英語
ページ(範囲)1-6
ページ数6
ジャーナルJournal of Cranio-Maxillofacial Surgery
42
1
DOI
出版ステータス出版済み - 1 2014
外部発表はい

All Science Journal Classification (ASJC) codes

  • 外科
  • 口腔外科
  • 耳鼻咽喉科学

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