TectoRNP: Self-assembling RNAs with peptide recognition motifs as templates for chemical peptide ligation

Kohei Yamashita, Takahiro Tanaka, Hiroyuki Furuta, Yoshiya Ikawa

    研究成果: Contribution to journalArticle査読

    2 被引用数 (Scopus)

    抄録

    TectoRNA, an artificial RNA with self-assembling ability, has been employed as a structural platform for RNA nanotechnology and RNA synthetic biology. In this study, tectoRNA was applied as a specific template for chemical peptide ligation. On the basis of a self-assembling tectoRNA, we designed and constructed a template RNA that facilitates peptide ligation depending on controlled dimer formation. Two RNA-binding peptides were recognized by two peptide-binding RNA motifs embedded in the template RNA, and chemical ligation was promoted because of the entropic effect of Mg2+-dependent dimerization. In a series of biochemical analyses, we determined the relationship between the structures of the tectoRNA-based templates and the extent of acceleration in peptide ligation.

    本文言語英語
    ページ(範囲)635-642
    ページ数8
    ジャーナルJournal of Peptide Science
    18
    10
    DOI
    出版ステータス出版済み - 10 2012

    All Science Journal Classification (ASJC) codes

    • 構造生物学
    • 生化学
    • 分子医療
    • 分子生物学
    • 薬理学
    • 創薬
    • 有機化学

    フィンガープリント

    「TectoRNP: Self-assembling RNAs with peptide recognition motifs as templates for chemical peptide ligation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル