Telomere-binding protein Taz1 controls global replication timing through its localization near late replication origins in fission yeast

Atsutoshi Tazumi, Masayoshi Fukuura, Ryuichiro Nakato, Ami Kishimoto, Tomokazu Takenaka, Shiho Ogawa, Ji Hoon Song, Tatsuro S. Takahashi, Takuro Nakagawa, Katsuhiko Shirahige, Hisao Masukata

研究成果: Contribution to journalArticle査読

62 被引用数 (Scopus)

抄録

In eukaryotes, the replication of chromosome DNA is coordinated by a replication timing program that temporally regulates the firing of individual replication origins. However, the molecular mechanism underlying the program remains elusive. Here, we report that the telomere-binding protein Taz1 plays a crucial role in the control of replication timing in fission yeast. A DNA element located proximal to a late origin in the chromosome arm represses initiation from the origin in early S phase. Systematic deletion and substitution experiments demonstrated that two tandem telomeric repeats are essential for this repression. The telomeric repeats recruit Taz1, a counterpart of human TRF1 and TRF2, to the locus. Genome-wide analysis revealed that Taz1 regulates about half of chromosomal late origins, including those in subtelomeres. The Taz1-mediated mechanism prevents Dbf4-dependent kinase (DDK)-dependent Sld3 loading onto the origins. Our results demonstrate that the replication timing program in fission yeast uses the internal telomeric repeats and binding of Taz1.

本文言語英語
ページ(範囲)2050-2062
ページ数13
ジャーナルGenes and Development
26
18
DOI
出版ステータス出版済み - 9 15 2012

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

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