Temperature response of photosynthesis and activities of phosphoenolpyruvate carboxylase and ribulose 1,5-bisphosphate carboxylase in greened calli induced from iceplant, Mesembryanthemum crystallinum L., leaves

Ken Ishimaru, Kazuyuki Saitou, Fumitake Kubota, Waichi Agata

研究成果: ジャーナルへの寄稿記事


Iceplant is a halophyte with two different photosynthetic modes of Ca and crassulacean acid metabolism (CAM). Environmental stress, such as a high salinity condition, is known as a trigger for shifting the photosynthetic mode from C 3 to CAM in a plant. The application of 6-benzylaminopurine to cell culture medium was effective to induce green calli from leaves of C 3 and CAM plants. The photosynthesis of greened calli obtained from C 3 and CAM plant leaves showed low temperature adaptation and wide temperature adaptation, respectively. In the calli derived from the C 3 plant, the activity of ribulose 1,5-bisphosphate carboxylase (RuBPCase) was higher than that of phosphoenolpyruvate carboxylase (PEPCase), while in the calli from the CAM plant, the activity of PEPCase was higher than that of RuBPCase. Thus, the greened calli showed the original levels of enzymatic activity in their donor plants. The activity of PEPCase in the greened calli obtained from the C 3 plant increased with the application of NaCl to the cell culture medium but this effect was not detected on nongreened calli.

ジャーナルJapanese Journal of Crop Science
出版物ステータス出版済み - 1 1 1997


All Science Journal Classification (ASJC) codes

  • Food Science
  • Agronomy and Crop Science
  • Genetics