Temperature-sensitive Arabidopsis mutant defective in 1-deoxy-D-xylulose 5-phosphate synthase within the plastid non-mevalonate pathway of isoprenoid biosynthesis

Naohiro Araki, Kensuke Kusumi, Kazumori Masamoto, Yasuo Niwa, Koh Iba

研究成果: ジャーナルへの寄稿記事

72 引用 (Scopus)

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The temperature-sensitive mutant of Arabidopsis, chs5, developed chlorotic leaves at restrictive temperatures (15 °C), but almost normal green leaves at permissive temperatures (22 °C). At the restrictive temperature, the chs5 mutation blocked the accumulation of chlorophylls and carotenoids. A temperature-shift analysis revealed that the manifestation of the chlorotic phenotype occurred in young leaf tissues, but did not in mature leaf tissues. Genetic and sequence analysis demonstrated that the chs5 mutation was caused by a single-base change in the coding region of a recently identified CLA1 gene. The CLA1 gene exhibited a high sequence similarity to the genes encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS) localized to the non-mevalonate pathway, which was recently discovered in bacteria and higher plants. In addition, the application of 1-deoxy-D-xylulose, the free sugar of 1-deoxy-D-xylulose 5-phosphate, rescues the defect in the chs5 mutant. These results indicated that the chlorotic phenotype of the chs5 mutant was caused by a defect in DXS activity and that DXS functions preferentially at an early stage of leaf cell development. A transiently expressed green fluorescent protein fused with the CLA1 transit peptide was localized within the chloroplasts in the green cultured cells of tobacco, which suggests that the putative localization of the non-mevalonate pathway is in plastids.

元の言語英語
ページ(範囲)19-24
ページ数6
ジャーナルPhysiologia Plantarum
108
発行部数1
DOI
出版物ステータス出版済み - 1 1 2000

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xylulose
Plastids
isoprenoids
Terpenes
Arabidopsis
plastids
biosynthesis
phosphates
mutants
Temperature
leaves
temperature
Xylulose
Genes
Phenotype
mutation
phenotype
genes
Chloroplasts
Carotenoids

All Science Journal Classification (ASJC) codes

  • Physiology
  • Genetics
  • Plant Science
  • Cell Biology

これを引用

Temperature-sensitive Arabidopsis mutant defective in 1-deoxy-D-xylulose 5-phosphate synthase within the plastid non-mevalonate pathway of isoprenoid biosynthesis. / Araki, Naohiro; Kusumi, Kensuke; Masamoto, Kazumori; Niwa, Yasuo; Iba, Koh.

:: Physiologia Plantarum, 巻 108, 番号 1, 01.01.2000, p. 19-24.

研究成果: ジャーナルへの寄稿記事

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abstract = "The temperature-sensitive mutant of Arabidopsis, chs5, developed chlorotic leaves at restrictive temperatures (15 °C), but almost normal green leaves at permissive temperatures (22 °C). At the restrictive temperature, the chs5 mutation blocked the accumulation of chlorophylls and carotenoids. A temperature-shift analysis revealed that the manifestation of the chlorotic phenotype occurred in young leaf tissues, but did not in mature leaf tissues. Genetic and sequence analysis demonstrated that the chs5 mutation was caused by a single-base change in the coding region of a recently identified CLA1 gene. The CLA1 gene exhibited a high sequence similarity to the genes encoding 1-deoxy-D-xylulose 5-phosphate synthase (DXS) localized to the non-mevalonate pathway, which was recently discovered in bacteria and higher plants. In addition, the application of 1-deoxy-D-xylulose, the free sugar of 1-deoxy-D-xylulose 5-phosphate, rescues the defect in the chs5 mutant. These results indicated that the chlorotic phenotype of the chs5 mutant was caused by a defect in DXS activity and that DXS functions preferentially at an early stage of leaf cell development. A transiently expressed green fluorescent protein fused with the CLA1 transit peptide was localized within the chloroplasts in the green cultured cells of tobacco, which suggests that the putative localization of the non-mevalonate pathway is in plastids.",
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