Temporal and spatial differential expression of chicken germlinespecific proteins cDAZL, CDH and CVH during gametogenesis

Gakushi Kito, Shinya Aramaki, Koji Tanaka, Tomoki Soh, Nobuhiko Yamauchi, Masa Aki Hattori

研究成果: ジャーナルへの寄稿記事

17 引用 (Scopus)

抄録

The Deleted in Azoospermia-Like (DAZL) protein coded by Dazl gene is a germline-specific RNA-binding protein essential for gametogenesis in vertebrates, and the chicken Dazl gene has also been identified in primordial germ cells (PGCs). However, the temporal and spatial expression of chicken DAZL (cDAZL) and its molecular role in germ cell development remain enigmatic. Here, we investigated the subcellular distribution and expression of cDAZL at the various stages by using a polyclonal antibody raised against its C-terminal region and compared them with those of additional germline-specific proteins chicken vasa homologue (CVH) and chicken dead end homologue (CDH). Western blot analysis for cDAZL revealed a single band in the embryonic gonads and premature chicken testis, whereas no band was detected in the premature chicken ovary. Fluorescent immunohistochemistry revealed that cDAZL was present in the nucleus and cytoplasm of circulating PGCs. Cells positive for cDAZL and CVH coexisted in the embryonic gonads and premature chicken testis, in which they were distributed near the basement membrane of seminiferous tubules. Of interest, cDAZL was not found in the premature chicken ovary, whereas CVH and CDH were present in germ cells. Collectively, three germline-specific proteins are expressed in chicken germ cells, but their patterns of expression are temporally and spatially distinct.

元の言語英語
ページ(範囲)341-346
ページ数6
ジャーナルJournal of Reproduction and Development
56
発行部数3
DOI
出版物ステータス出版済み - 6 1 2010

Fingerprint

gametogenesis
chickens
germ cells
proteins
gonads
testes
RNA-binding proteins
seminiferous tubules
basement membrane
polyclonal antibodies

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

これを引用

Temporal and spatial differential expression of chicken germlinespecific proteins cDAZL, CDH and CVH during gametogenesis. / Kito, Gakushi; Aramaki, Shinya; Tanaka, Koji; Soh, Tomoki; Yamauchi, Nobuhiko; Hattori, Masa Aki.

:: Journal of Reproduction and Development, 巻 56, 番号 3, 01.06.2010, p. 341-346.

研究成果: ジャーナルへの寄稿記事

@article{4fee974f37d54171bb3d662c669b356f,
title = "Temporal and spatial differential expression of chicken germlinespecific proteins cDAZL, CDH and CVH during gametogenesis",
abstract = "The Deleted in Azoospermia-Like (DAZL) protein coded by Dazl gene is a germline-specific RNA-binding protein essential for gametogenesis in vertebrates, and the chicken Dazl gene has also been identified in primordial germ cells (PGCs). However, the temporal and spatial expression of chicken DAZL (cDAZL) and its molecular role in germ cell development remain enigmatic. Here, we investigated the subcellular distribution and expression of cDAZL at the various stages by using a polyclonal antibody raised against its C-terminal region and compared them with those of additional germline-specific proteins chicken vasa homologue (CVH) and chicken dead end homologue (CDH). Western blot analysis for cDAZL revealed a single band in the embryonic gonads and premature chicken testis, whereas no band was detected in the premature chicken ovary. Fluorescent immunohistochemistry revealed that cDAZL was present in the nucleus and cytoplasm of circulating PGCs. Cells positive for cDAZL and CVH coexisted in the embryonic gonads and premature chicken testis, in which they were distributed near the basement membrane of seminiferous tubules. Of interest, cDAZL was not found in the premature chicken ovary, whereas CVH and CDH were present in germ cells. Collectively, three germline-specific proteins are expressed in chicken germ cells, but their patterns of expression are temporally and spatially distinct.",
author = "Gakushi Kito and Shinya Aramaki and Koji Tanaka and Tomoki Soh and Nobuhiko Yamauchi and Hattori, {Masa Aki}",
year = "2010",
month = "6",
day = "1",
doi = "10.1262/jrd.09-218A",
language = "English",
volume = "56",
pages = "341--346",
journal = "Journal of Reproduction and Development",
issn = "0916-8818",
publisher = "Japanese Society of Animal Reproduction (JSAR)",
number = "3",

}

TY - JOUR

T1 - Temporal and spatial differential expression of chicken germlinespecific proteins cDAZL, CDH and CVH during gametogenesis

AU - Kito, Gakushi

AU - Aramaki, Shinya

AU - Tanaka, Koji

AU - Soh, Tomoki

AU - Yamauchi, Nobuhiko

AU - Hattori, Masa Aki

PY - 2010/6/1

Y1 - 2010/6/1

N2 - The Deleted in Azoospermia-Like (DAZL) protein coded by Dazl gene is a germline-specific RNA-binding protein essential for gametogenesis in vertebrates, and the chicken Dazl gene has also been identified in primordial germ cells (PGCs). However, the temporal and spatial expression of chicken DAZL (cDAZL) and its molecular role in germ cell development remain enigmatic. Here, we investigated the subcellular distribution and expression of cDAZL at the various stages by using a polyclonal antibody raised against its C-terminal region and compared them with those of additional germline-specific proteins chicken vasa homologue (CVH) and chicken dead end homologue (CDH). Western blot analysis for cDAZL revealed a single band in the embryonic gonads and premature chicken testis, whereas no band was detected in the premature chicken ovary. Fluorescent immunohistochemistry revealed that cDAZL was present in the nucleus and cytoplasm of circulating PGCs. Cells positive for cDAZL and CVH coexisted in the embryonic gonads and premature chicken testis, in which they were distributed near the basement membrane of seminiferous tubules. Of interest, cDAZL was not found in the premature chicken ovary, whereas CVH and CDH were present in germ cells. Collectively, three germline-specific proteins are expressed in chicken germ cells, but their patterns of expression are temporally and spatially distinct.

AB - The Deleted in Azoospermia-Like (DAZL) protein coded by Dazl gene is a germline-specific RNA-binding protein essential for gametogenesis in vertebrates, and the chicken Dazl gene has also been identified in primordial germ cells (PGCs). However, the temporal and spatial expression of chicken DAZL (cDAZL) and its molecular role in germ cell development remain enigmatic. Here, we investigated the subcellular distribution and expression of cDAZL at the various stages by using a polyclonal antibody raised against its C-terminal region and compared them with those of additional germline-specific proteins chicken vasa homologue (CVH) and chicken dead end homologue (CDH). Western blot analysis for cDAZL revealed a single band in the embryonic gonads and premature chicken testis, whereas no band was detected in the premature chicken ovary. Fluorescent immunohistochemistry revealed that cDAZL was present in the nucleus and cytoplasm of circulating PGCs. Cells positive for cDAZL and CVH coexisted in the embryonic gonads and premature chicken testis, in which they were distributed near the basement membrane of seminiferous tubules. Of interest, cDAZL was not found in the premature chicken ovary, whereas CVH and CDH were present in germ cells. Collectively, three germline-specific proteins are expressed in chicken germ cells, but their patterns of expression are temporally and spatially distinct.

UR - http://www.scopus.com/inward/record.url?scp=77954259658&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77954259658&partnerID=8YFLogxK

U2 - 10.1262/jrd.09-218A

DO - 10.1262/jrd.09-218A

M3 - Article

C2 - 20332590

AN - SCOPUS:77954259658

VL - 56

SP - 341

EP - 346

JO - Journal of Reproduction and Development

JF - Journal of Reproduction and Development

SN - 0916-8818

IS - 3

ER -