Tertiary structure of mouse epidermal growth factor determined by two-dimensional 1H NMR

Daisuke Kohda, Nobuhiro Go, Kyozo Hayashi, Fuyuhiko Inagaki

研究成果: Contribution to journalArticle査読

45 被引用数 (Scopus)

抄録

The tertiary structure of mouse epidermal growth factor (EGF) in solution (28°C, pH 2.0) was studied by two-dimensional NMR spectroscopy. Proton-proton distance constraints derived from NOESY spectra were used to construct a mechanical molecular model of mouse EGF, which was subsequently checked by means of a preliminary distance geometry calculation. The chain-folds in the two structural domains of mouse EGF were very similar to those previously reported (Montelione et al. (1987) Proc. Natl. Acad. Sci. U.S. 84, 5226-5230). However, the relative orientations of the two domains were different. Because we could assign much more inter-domain NOEs, the relative orientations of the two domains were well determined in our model. The hollow between the two domains may function as a binding site for the EGF receptor.

本文言語英語
ページ(範囲)741-743
ページ数3
ジャーナルJournal of biochemistry
103
5
DOI
出版ステータス出版済み - 5 1988
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学
  • 分子生物学

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