The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining

Ryuji Hiramatsu, Kiwamu Akagi, Masao Matsuoka, Kunihiko Sakumi, Hiro Nakamura, Linda Kingsbury, Chella David, Richard R. Hardy, Ken ichi Yamamura, Hitoshi Sakano

研究成果: Contribution to journalArticle査読

57 被引用数 (Scopus)

抄録

Using transgenic substrates, we found that the immunoglobulin κ gene 3′ enhancer (E3′) acts as a negative regulator in Vκ-Jκ joining. Although the E3′ was originally identified as a transcriptional enhancer, it acts in a Buppressive manner for recombinational regulation. Base substitution analysis has shown that the PU. 1-binding site within the E3′ regulates the B/T specificity of Vκ-Jκ joining. In a substrate with a mutated PU. 1-binding site (GAGGAA to TCTTCG), Vκ-Jκ joining occurred not only in B cells, but also in T cells. The E3′ region is also responsible for determining the pro-B/pre-B specificity of Vκ-Jκ joining. When the E3′ region was deleted, κ gene rearrangement actively occurred at the early pro-B stage of B cell development: non-germline (N) nucleotides were common at recombination junctions.

本文言語英語
ページ(範囲)1113-1123
ページ数11
ジャーナルCell
83
7
DOI
出版ステータス出版済み - 12 29 1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • 生化学、遺伝学、分子生物学(全般)

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「The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin V<sub>κ</sub>-J<sub>κ</sub> joining」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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