The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining

Ryuji Hiramatsu, Kiwamu Akagi, Masao Matsuoka, Kunihiko Sakumi, Hiro Nakamura, Linda Kingsbury, Chella David, Richard R. Hardy, Ken ichi Yamamura, Hitoshi Sakano

研究成果: ジャーナルへの寄稿記事

56 引用 (Scopus)

抜粋

Using transgenic substrates, we found that the immunoglobulin κ gene 3′ enhancer (E3′) acts as a negative regulator in Vκ-Jκ joining. Although the E3′ was originally identified as a transcriptional enhancer, it acts in a Buppressive manner for recombinational regulation. Base substitution analysis has shown that the PU. 1-binding site within the E3′ regulates the B/T specificity of Vκ-Jκ joining. In a substrate with a mutated PU. 1-binding site (GAGGAA to TCTTCG), Vκ-Jκ joining occurred not only in B cells, but also in T cells. The E3′ region is also responsible for determining the pro-B/pre-B specificity of Vκ-Jκ joining. When the E3′ region was deleted, κ gene rearrangement actively occurred at the early pro-B stage of B cell development: non-germline (N) nucleotides were common at recombination junctions.

元の言語英語
ページ(範囲)1113-1123
ページ数11
ジャーナルCell
83
発行部数7
DOI
出版物ステータス出版済み - 12 29 1995
外部発表Yes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

フィンガープリント The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin V<sub>κ</sub>-J<sub>κ</sub> joining' の研究トピックを掘り下げます。これらはともに一意のフィンガープリントを構成します。

  • これを引用

    Hiramatsu, R., Akagi, K., Matsuoka, M., Sakumi, K., Nakamura, H., Kingsbury, L., David, C., Hardy, R. R., Yamamura, K. I., & Sakano, H. (1995). The 3′ enhancer region determines the B/T specificity and pro-B/pre-B specificity of immunoglobulin Vκ-Jκ joining. Cell, 83(7), 1113-1123. https://doi.org/10.1016/0092-8674(95)90138-8