The 5′-region of the P450XIA1 (P450scc) gene contains a basal promoter and an adrenal-specific activating domain

Bon chu Chung, Meng Chun Hu, Char Chang Lai, Cheng Hui Lin

研究成果: Contribution to journalArticle査読

25 被引用数 (Scopus)

抄録

The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5′-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcriptionaby two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145 -573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.

本文言語英語
ページ(範囲)276-281
ページ数6
ジャーナルBiochemical and Biophysical Research Communications
160
1
DOI
出版ステータス出版済み - 4 14 1989

All Science Journal Classification (ASJC) codes

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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