抄録
The first step to the synthesis of all steroids is catalyzed by P450scc. We constructed nine deletion mutants of the 5′-region of the P450scc gene and connected them to a CAT reporter gene to assay transcriptional activity of the P450scc promoter. A short 145 bp fragment stimulated transcriptionaby two fold. This DNA was active in all cells tested irrespective of their tissue origin and steroidogenic activity. DNA at -145 -573 of the upstream region did not increase transcription any further. DNA including 2500 bp of the upstream region stimulated transcription by 10 fold only in adrenal Y-1 cells. Hence in the -145 region contains a low level P450scc promoter and the 2500 bp DNA possesses an adrenal specific enhancing element.
本文言語 | 英語 |
---|---|
ページ(範囲) | 276-281 |
ページ数 | 6 |
ジャーナル | Biochemical and Biophysical Research Communications |
巻 | 160 |
号 | 1 |
DOI | |
出版ステータス | 出版済み - 4月 14 1989 |
!!!All Science Journal Classification (ASJC) codes
- 生物理学
- 生化学
- 分子生物学
- 細胞生物学