The amino acid sequence required for 5' → 3' exonuclease activity of bacillus caldotenax DNA polymerase

Yoshizumi Ishino, Asako Takahashi-Fujii, Takashi Uemori, Mitsuo Imamura, Ikunoshin Kato

研究成果: Contribution to journalArticle査読

5 被引用数 (Scopus)

抄録

We studied the 5' ↑ 3' exonuclease activity of Bacillus caldotenax DNA polymerase by site-directed mutagenesis. Among seven mutants constructed, two mutant DNA polymerases with an amino acid substitution of Glyl84 ↑ Asp or Glyl92 ↑ Asp were confirmed to be deficient in this exonuclease. The two positions corresponded to those of the Escherichia coli DNA polymerase I mutants defective in 5' ↑ 3' exonuclease, polA480ex and polA214. These results provide experimental support for the proposed amino acid sequence essential for the 5' ↑ 3' exonuclease activity associated with eubacterial polymerase I-like DNA polymerases (family A), including E.coli and Thermits aquaticus.

本文言語英語
ページ(範囲)1171-1175
ページ数5
ジャーナルProtein Engineering, Design and Selection
8
11
DOI
出版ステータス出版済み - 11 1 1995
外部発表はい

All Science Journal Classification (ASJC) codes

  • バイオテクノロジー
  • バイオエンジニアリング
  • 生化学
  • 分子生物学

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