The B lymphocyte adaptor molecule of 32 kilodaltons (Bam32) regulates B cell antigen receptor internalization

Hiroaki Niiro, Atef Allam, Angela Stoddart, Frances M. Brodsky, Aaron J. Marshall, Edward A. Clark

研究成果: ジャーナルへの寄稿記事

47 引用 (Scopus)

抄録

The B lymphocyte adaptor molecule of 32 kDa (Bam32) is an adaptor that plays an indispensable role in BCR signaling. In this study, we found that upon BCR ligation, Bam32 is recruited to the plasma membrane where it associates with BCR complexes and redistributes and internalizes with BCRs. BCR ligation induced colocalization of Bam32 with lipid rafts, clathrin, and actin filaments. An inhibitor of Src family protein tyrosine kinases (PTKs) blocked both BCR-inctaced tyrosine phosphorylation of Bam32 and BCR internalization. Moreover, BCR internalization is impaired in Bam32-/- and Lyn -/- cells, and expression of Bam32 with a mutation of its tyrosine phosphorylation site (Y139F) inhibited BCR internalization. These data suggest that Bam32 functions downstream of Src family PTKs to regulate BCR internalization. Bam32 deficiency does not affect tyrosine phosphorylation of clathrin or the association of clathrin with lipid rafts upon BCR cross-linking. However, BCR-induced actin polymerization is impaired in Bam32-/- cells. Collectively, these findings indicate a novel role of Bam32 in connecting Src family PTKs to BCR internalization by an actin-dependent mechanism.

元の言語英語
ページ(範囲)5601-5609
ページ数9
ジャーナルJournal of Immunology
173
発行部数9
DOI
出版物ステータス出版済み - 11 1 2004
外部発表Yes

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B-Cell Antigen Receptors
B-Lymphocytes
src-Family Kinases
Clathrin
Tyrosine
Phosphorylation
Ligation
Actins
Lipids
Actin Cytoskeleton
Polymerization
Cell Membrane

All Science Journal Classification (ASJC) codes

  • Immunology

これを引用

The B lymphocyte adaptor molecule of 32 kilodaltons (Bam32) regulates B cell antigen receptor internalization. / Niiro, Hiroaki; Allam, Atef; Stoddart, Angela; Brodsky, Frances M.; Marshall, Aaron J.; Clark, Edward A.

:: Journal of Immunology, 巻 173, 番号 9, 01.11.2004, p. 5601-5609.

研究成果: ジャーナルへの寄稿記事

Niiro, Hiroaki ; Allam, Atef ; Stoddart, Angela ; Brodsky, Frances M. ; Marshall, Aaron J. ; Clark, Edward A. / The B lymphocyte adaptor molecule of 32 kilodaltons (Bam32) regulates B cell antigen receptor internalization. :: Journal of Immunology. 2004 ; 巻 173, 番号 9. pp. 5601-5609.
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