The characteristic analysis of the specific interaction between PDZ-domain and its recognition peptide by dynamic force spectroscopy

Tei Maki, Satoru Kidoaki, Takehisa Matsuda, Kengo Usui, Harukazu Suzuki, Masayoshi Ito, Yoshihide Hayashizaki

研究成果: 会議への寄与タイプ論文

抄録

To characterize the specific interaction between PDZ-protein (TIP-1) and its recognition peptide (PDZ-pep), dynamic force spectroscopy (DPS) for these pair was performed using AFM. Unbinding forces of these pair were measured under different conditions of AFM tip retracting velocity. The unbinding force spectra on logarithmic force-loading rate exhibited two different rate regimes each of where the forces linearly increased with the logarithmic rate. Based on DFS theory, positions of two different activation barriers in reaction coordinate and dissociation rate constants in each barrier were evaluated from slopes and x-intercepts of the two linear regimes. Two-step dissociation kinetics between TIP-1 and PDZ-pep was suggested.

元の言語英語
ページ3231-3232
ページ数2
出版物ステータス出版済み - 12 1 2005
イベント54th SPSJ Symposium on Macromolecules - Yamagata, 日本
継続期間: 9 20 20059 22 2005

その他

その他54th SPSJ Symposium on Macromolecules
日本
Yamagata
期間9/20/059/22/05

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Peptides
Spectroscopy
Rate constants
Chemical activation
Proteins
Kinetics

All Science Journal Classification (ASJC) codes

  • Engineering(all)

これを引用

Maki, T., Kidoaki, S., Matsuda, T., Usui, K., Suzuki, H., Ito, M., & Hayashizaki, Y. (2005). The characteristic analysis of the specific interaction between PDZ-domain and its recognition peptide by dynamic force spectroscopy. 3231-3232. 論文発表場所 54th SPSJ Symposium on Macromolecules, Yamagata, 日本.

The characteristic analysis of the specific interaction between PDZ-domain and its recognition peptide by dynamic force spectroscopy. / Maki, Tei; Kidoaki, Satoru; Matsuda, Takehisa; Usui, Kengo; Suzuki, Harukazu; Ito, Masayoshi; Hayashizaki, Yoshihide.

2005. 3231-3232 論文発表場所 54th SPSJ Symposium on Macromolecules, Yamagata, 日本.

研究成果: 会議への寄与タイプ論文

Maki, T, Kidoaki, S, Matsuda, T, Usui, K, Suzuki, H, Ito, M & Hayashizaki, Y 2005, 'The characteristic analysis of the specific interaction between PDZ-domain and its recognition peptide by dynamic force spectroscopy' 論文発表場所 54th SPSJ Symposium on Macromolecules, Yamagata, 日本, 9/20/05 - 9/22/05, pp. 3231-3232.
Maki T, Kidoaki S, Matsuda T, Usui K, Suzuki H, Ito M その他. The characteristic analysis of the specific interaction between PDZ-domain and its recognition peptide by dynamic force spectroscopy. 2005. 論文発表場所 54th SPSJ Symposium on Macromolecules, Yamagata, 日本.
Maki, Tei ; Kidoaki, Satoru ; Matsuda, Takehisa ; Usui, Kengo ; Suzuki, Harukazu ; Ito, Masayoshi ; Hayashizaki, Yoshihide. / The characteristic analysis of the specific interaction between PDZ-domain and its recognition peptide by dynamic force spectroscopy. 論文発表場所 54th SPSJ Symposium on Macromolecules, Yamagata, 日本.2 p.
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