The clinical significance of vimentin-expressing gastric cancer cells in bone marrow

Masaaki Iwatsuki, Koshi Mimori, Takeo Fukagawa, Hideshi Ishii, Takehiko Yokobori, Mitsuru Sasako, Hideo Baba, Masaki Mori

研究成果: ジャーナルへの寄稿記事

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Background: Expression of the mesenchymal marker gene vimentin (VIM) in gastric cancer is associated with a more aggressive form of the disease and poor prognosis. Because epithelial mesenchymal transition (EMT) plays a critical role in the progression of gastric cancer, VIM expression was examined in the bone marrow (BM) of gastric cancer patients. Methods: BM samples from 437 gastric cancer patients were collected and analyzed by quantitative RT-PCR. Expression of VIM protein in the primary lesions of resected gastric cancers was evaluated using immunohistochemistry. Furthermore, induction of VIM expression by TGF-β1 and hypoxia was evaluated in gastric cancer cells. Results: VIM mRNA expression increased concordantly with clinical staging and was significantly associated with tumor invasion and lymph node metastasis (P < .0001). Though cancer cells in the primary lesions did not stain with VIM antibody, some of the cells invading the intratumoral vessels were strongly positive for VIM, but were negative for E-cadherin. Hypoxic conditions and treatment with TGF-β1 induced VIM expression and repressed E-cadherin in gastric cancer cells, coupled with an alteration of cellular morphology. Conclusions: We found that gastric cancer cells undergo EMT in BM to survive and metastasize. These findings suggest that isolated tumor cells have the potential to undergo EMT, which could increase the malignancy of gastric cancer.

元の言語英語
ページ(範囲)2526-2533
ページ数8
ジャーナルAnnals of Surgical Oncology
17
発行部数9
DOI
出版物ステータス出版済み - 9 1 2010

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All Science Journal Classification (ASJC) codes

  • Surgery
  • Oncology

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