The Complete Amino Acid Sequence of Antiviral Protein from the Seeds of Pokeweed (Phytolacca americana)

Sung Sil Kung, Makoto Kimura, Gunki Funatsu

研究成果: ジャーナルへの寄稿記事

28 引用 (Scopus)

抄録

The complete amino acid sequence of antiviral protein from the seeds of pokeweed (Phytolacca americana) has been determined. This has been done by the sequence analysis of peptides derived by enzymatic digestion with trypsin, pepsin, and Iysylendopeptidase, as well as by chemical cleavage with cyanogen bromide. The protein consists of 261 amino acid residues containing two disulfide bonds and has a calculated molecular mass of 29167 Da. The two disulfide bonds connect Cys-34 to Cys-258 and Cys-84 to Cys-105. Comparison of this sequence with the sequence of the ricin A-chain shows that there are identical residues at 76 positions in the two molecules (30% identity), having an extended region of highly conserved sequence at positions 170-183 (IQMXSEAARFXYIE). In contrast, the internal regions at positions 77 119 and 141 169 and the C-terminal 15 amino acid residues are less homologous in the two proteins.

元の言語英語
ページ(範囲)3301-3318
ページ数18
ジャーナルAgricultural and Biological Chemistry
54
発行部数12
DOI
出版物ステータス出版済み - 1 1 1990

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antiviral proteins
Phytolacca americana
disulfide bonds
Disulfides
Amino Acid Sequence
amino acid sequences
ricin
cyanogen
Ricin
Amino Acids
Cyanogen Bromide
amino acids
conserved sequences
Conserved Sequence
Pepsin A
Protein Sequence Analysis
pepsin
seeds
bromides
Trypsin

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

The Complete Amino Acid Sequence of Antiviral Protein from the Seeds of Pokeweed (Phytolacca americana). / Kung, Sung Sil; Kimura, Makoto; Funatsu, Gunki.

:: Agricultural and Biological Chemistry, 巻 54, 番号 12, 01.01.1990, p. 3301-3318.

研究成果: ジャーナルへの寄稿記事

Kung, Sung Sil ; Kimura, Makoto ; Funatsu, Gunki. / The Complete Amino Acid Sequence of Antiviral Protein from the Seeds of Pokeweed (Phytolacca americana). :: Agricultural and Biological Chemistry. 1990 ; 巻 54, 番号 12. pp. 3301-3318.
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