抄録
The complete amino acid sequence of rye seed chitinase-c (RSC-c) has been analyzed. This was done by first sequencing the tryptic peptides from RCm-RSC-c and then connecting them by analyzing the peptides produced by digestions with lysylendopeptidase and Staphylococcus aureus V8 protease of RCm-RSC-c, and by chymotryptic digestion and formic acid cleavage of S. aureus V8 protease peptides. RSC-c consists of 243 amino acid residues and has a molecular mass of 26, 093, and has 92% sequence homology with barley seed basic chitinase which lacks a Cys-rich domain. Cys204 is free and six cysteine residues are linked by disulfide bonds between Cys23 and Cys85, Cys97 and Cys105, and Cys223 and Cys236.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 1854-1861 |
| ページ数 | 8 |
| ジャーナル | Bioscience, Biotechnology, and Biochemistry |
| 巻 | 57 |
| 発行部数 | 11 |
| DOI | |
| 出版物ステータス | 出版済み - 1 1 1993 |
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All Science Journal Classification (ASJC) codes
- Biotechnology
- Analytical Chemistry
- Biochemistry
- Applied Microbiology and Biotechnology
- Molecular Biology
- Organic Chemistry
これを引用
The Complete Amino Acid Sequence of Chitinase-c from the Seeds of Rye (Secale cereal). / Yamagami, Takeshi; Funatsu, Gunki.
：: Bioscience, Biotechnology, and Biochemistry, 巻 57, 番号 11, 01.01.1993, p. 1854-1861.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - The Complete Amino Acid Sequence of Chitinase-c from the Seeds of Rye (Secale cereal)
AU - Yamagami, Takeshi
AU - Funatsu, Gunki
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The complete amino acid sequence of rye seed chitinase-c (RSC-c) has been analyzed. This was done by first sequencing the tryptic peptides from RCm-RSC-c and then connecting them by analyzing the peptides produced by digestions with lysylendopeptidase and Staphylococcus aureus V8 protease of RCm-RSC-c, and by chymotryptic digestion and formic acid cleavage of S. aureus V8 protease peptides. RSC-c consists of 243 amino acid residues and has a molecular mass of 26, 093, and has 92% sequence homology with barley seed basic chitinase which lacks a Cys-rich domain. Cys204 is free and six cysteine residues are linked by disulfide bonds between Cys23 and Cys85, Cys97 and Cys105, and Cys223 and Cys236.
AB - The complete amino acid sequence of rye seed chitinase-c (RSC-c) has been analyzed. This was done by first sequencing the tryptic peptides from RCm-RSC-c and then connecting them by analyzing the peptides produced by digestions with lysylendopeptidase and Staphylococcus aureus V8 protease of RCm-RSC-c, and by chymotryptic digestion and formic acid cleavage of S. aureus V8 protease peptides. RSC-c consists of 243 amino acid residues and has a molecular mass of 26, 093, and has 92% sequence homology with barley seed basic chitinase which lacks a Cys-rich domain. Cys204 is free and six cysteine residues are linked by disulfide bonds between Cys23 and Cys85, Cys97 and Cys105, and Cys223 and Cys236.
UR - http://www.scopus.com/inward/record.url?scp=85007857366&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85007857366&partnerID=8YFLogxK
U2 - 10.1271/bbb.57.1854
DO - 10.1271/bbb.57.1854
M3 - Article
C2 - 7764335
AN - SCOPUS:85007857366
VL - 57
SP - 1854
EP - 1861
JO - Bioscience, Biotechnology and Biochemistry
JF - Bioscience, Biotechnology and Biochemistry
SN - 0916-8451
IS - 11
ER -