The Complete Amino Acid Sequence of Chitinase-c from the Seeds of Rye (Secale cereal)

Takeshi Yamagami, Gunki Funatsu

研究成果: ジャーナルへの寄稿記事

27 引用 (Scopus)

抄録

The complete amino acid sequence of rye seed chitinase-c (RSC-c) has been analyzed. This was done by first sequencing the tryptic peptides from RCm-RSC-c and then connecting them by analyzing the peptides produced by digestions with lysylendopeptidase and Staphylococcus aureus V8 protease of RCm-RSC-c, and by chymotryptic digestion and formic acid cleavage of S. aureus V8 protease peptides. RSC-c consists of 243 amino acid residues and has a molecular mass of 26, 093, and has 92% sequence homology with barley seed basic chitinase which lacks a Cys-rich domain. Cys204 is free and six cysteine residues are linked by disulfide bonds between Cys23 and Cys85, Cys97 and Cys105, and Cys223 and Cys236.

元の言語英語
ページ(範囲)1854-1861
ページ数8
ジャーナルBioscience, Biotechnology, and Biochemistry
57
発行部数11
DOI
出版物ステータス出版済み - 1 1 1993

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Chitinases
Seed
Amino Acid Sequence
Seeds
Amino Acids
formic acid
Peptides
Staphylococcus aureus
Digestion
Molecular mass
Hordeum
Sequence Homology
Disulfides
Cysteine
Secale
Edible Grain

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

The Complete Amino Acid Sequence of Chitinase-c from the Seeds of Rye (Secale cereal). / Yamagami, Takeshi; Funatsu, Gunki.

:: Bioscience, Biotechnology, and Biochemistry, 巻 57, 番号 11, 01.01.1993, p. 1854-1861.

研究成果: ジャーナルへの寄稿記事

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