The complete amino acid sequence of ribonuclease from the seeds of bitter gourd (Momordica charantia)

Hiroyuki Ide, Makoto Kimura, Mariko Arai, Gunki Funatsu

研究成果: Contribution to journalArticle査読

69 被引用数 (Scopus)

抄録

The complete amino acid sequence of ribonuclease (RNase MC) from the seeds of bitter gourd (Momordica charantia) has been determined. This has been achieved by the sequence analysis of peptides derived by enzymatic digestion with trypsin, lysylendopeptidase, and chymotrypsin, as well as by chemical cleavage with cyanogen bromide. The protein contains 191 amino acid residues and has a calculated molecular mass or 21 259 Da. Comparison of this sequence with sequences of the fungal RNases, RNase T2, and RNase Rh, revealed that there are highly conserved residues at positions 32-38 (TXHGLWP) and 81-92 (FWXHEWXKHGTC). Furthermore, the sequence of RNase MC was found to be homologous to those of Nicotiana alata S-glycoproteins involved in self-incompatibility sharing 41% identical residues.

本文言語英語
ページ(範囲)161-164
ページ数4
ジャーナルFEBS Letters
284
2
DOI
出版ステータス出版済み - 6 24 1991

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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