The Complete Amino Acid Sequences of the B-Chains of Abrin-a and Abrin-b, Toxic Proteins from the Seeds of Abrus Precatorius

Makoto Kimura, Tomoyuki Sumizawa, Gunki Funatsu

研究成果: ジャーナルへの寄稿記事

21 引用 (Scopus)

抄録

The amino acids of the B-chains of two abrins (designated as abrin-a and abrin-b) from the seeds of Abrus precatorius have been sequenced. The sequence of the B-chain of abrin-a was solved by analysis of peptides derived by enzymatic digestions with trypsin, lysylendopeptidase, and chymotrypsin, as well as by chemical cleavage with cyanogen bromide. The sequence of the B-chain of abrin-b was analyzed by sequence analysis of tryptic peptides and comparing these sequences with those of corresponding peptides of the B-chain of abrin-a. The B-chains of abrin-a and abrin-b consist of 268 amino acid residues and share 256 identical residues. Comparison of their sequences with that of the ricin B-chain shows that 60% of the residues of both abrin B-chains are identical to those of the ricin B-chain and that two saccharide-binding sites in ricin B-chain identified by a crystallographic study are highly conserved in both abrin B-chains.

元の言語英語
ページ(範囲)166-169
ページ数4
ジャーナルBioscience, biotechnology, and biochemistry
57
発行部数1
DOI
出版物ステータス出版済み - 1 1 1993

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Abrin
Abrus
Poisons
Seed
Amino Acid Sequence
Seeds
Amino Acids
Proteins
Ricin
Cyanogen Bromide
Peptides
Protein Sequence Analysis
Digestion

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

The Complete Amino Acid Sequences of the B-Chains of Abrin-a and Abrin-b, Toxic Proteins from the Seeds of Abrus Precatorius. / Kimura, Makoto; Sumizawa, Tomoyuki; Funatsu, Gunki.

:: Bioscience, biotechnology, and biochemistry, 巻 57, 番号 1, 01.01.1993, p. 166-169.

研究成果: ジャーナルへの寄稿記事

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abstract = "The amino acids of the B-chains of two abrins (designated as abrin-a and abrin-b) from the seeds of Abrus precatorius have been sequenced. The sequence of the B-chain of abrin-a was solved by analysis of peptides derived by enzymatic digestions with trypsin, lysylendopeptidase, and chymotrypsin, as well as by chemical cleavage with cyanogen bromide. The sequence of the B-chain of abrin-b was analyzed by sequence analysis of tryptic peptides and comparing these sequences with those of corresponding peptides of the B-chain of abrin-a. The B-chains of abrin-a and abrin-b consist of 268 amino acid residues and share 256 identical residues. Comparison of their sequences with that of the ricin B-chain shows that 60{\%} of the residues of both abrin B-chains are identical to those of the ricin B-chain and that two saccharide-binding sites in ricin B-chain identified by a crystallographic study are highly conserved in both abrin B-chains.",
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