The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNa from Pyrococcus horikoshii OT3

Tadashi Hara, Atsushi Terada, Hiroki Yamaguchi, Takashi Nakashima, Yoshimitsu Kakuta, Makoto Kimura

研究成果: ジャーナルへの寄稿記事

4 引用 (Scopus)

抄録

We investigated the contribution of peripheral stem-loops to the catalytic activity of an archaeal RNase P RNA, PhopRNA, from Pyrococcus horikoshii OT3. PhopRNA mutants, in which the stem-loops were individually deleted, were prepared and characterized with respect to precursor tRNA (pre-tRNA) cleavage activity in the presence of five RNase P proteins. All the mutants retained the activity to some extent, indicating that they are moderately implicated in catalysis. Further characterization suggested that the stem-loops serve largely as binding sites for the proteins, and that their interactions are predominantly involved in stabilization of the active conformation of PhopRNA.

元の言語英語
ページ(範囲)816-819
ページ数4
ジャーナルBioscience, Biotechnology and Biochemistry
75
発行部数4
DOI
出版物ステータス出版済み - 5 16 2011

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Pyrococcus horikoshii
Ribonuclease P
Catalyst activity
RNA Precursors
Catalysis
Conformations
Carrier Proteins
Proteins
Stabilization
Binding Sites
RNA

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

これを引用

The contribution of peripheral stem-loops to the catalytic activity of archaeal RNase P RNa from Pyrococcus horikoshii OT3. / Hara, Tadashi; Terada, Atsushi; Yamaguchi, Hiroki; Nakashima, Takashi; Kakuta, Yoshimitsu; Kimura, Makoto.

:: Bioscience, Biotechnology and Biochemistry, 巻 75, 番号 4, 16.05.2011, p. 816-819.

研究成果: ジャーナルへの寄稿記事

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