TY - CHAP
T1 - The corneal micropocket assay
T2 - A model of angiogenesis and lymphangiogenesis
AU - Nakao, Shintaro
AU - Hafezi-Moghadam, Ali
PY - 2016/1/1
Y1 - 2016/1/1
N2 - The cornea is a transparent tissue that lacks blood and lymphatic vessels. In addition, the cornea is readily accessible, which makes it convenient for direct visualization of angiogenesis and lymphangiogenesis. The corneal micropocket assay is a commonly used quantitative technique, in which a growth factor containing pellet is micro-surgically implanted into the cornea of a rodent. Subsequently, the growth of the preexisting limbal vessels toward the growth factor is visualized by live microscopy or immunohistochemistry. Recently, there has been signifi cant interest in the process of lymphangiogenesis and the factors that regulate it. To facilitate these studies, we introduce a novel technique for visualization of the immune response during growth factor induced angiogenesis and lymphangiogenesis in the cornea.
AB - The cornea is a transparent tissue that lacks blood and lymphatic vessels. In addition, the cornea is readily accessible, which makes it convenient for direct visualization of angiogenesis and lymphangiogenesis. The corneal micropocket assay is a commonly used quantitative technique, in which a growth factor containing pellet is micro-surgically implanted into the cornea of a rodent. Subsequently, the growth of the preexisting limbal vessels toward the growth factor is visualized by live microscopy or immunohistochemistry. Recently, there has been signifi cant interest in the process of lymphangiogenesis and the factors that regulate it. To facilitate these studies, we introduce a novel technique for visualization of the immune response during growth factor induced angiogenesis and lymphangiogenesis in the cornea.
UR - http://www.scopus.com/inward/record.url?scp=84979284930&partnerID=8YFLogxK
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U2 - 10.1007/978-1-4939-3628-1_21
DO - 10.1007/978-1-4939-3628-1_21
M3 - Chapter
C2 - 27172963
AN - SCOPUS:84979284930
T3 - Methods in Molecular Biology
SP - 311
EP - 316
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -