The corneal micropocket assay: A model of angiogenesis and lymphangiogenesis

Shintaro Nakao, Ali Hafezi-Moghadam

研究成果: Chapter in Book/Report/Conference proceedingChapter

4 被引用数 (Scopus)

抄録

The cornea is a transparent tissue that lacks blood and lymphatic vessels. In addition, the cornea is readily accessible, which makes it convenient for direct visualization of angiogenesis and lymphangiogenesis. The corneal micropocket assay is a commonly used quantitative technique, in which a growth factor containing pellet is micro-surgically implanted into the cornea of a rodent. Subsequently, the growth of the preexisting limbal vessels toward the growth factor is visualized by live microscopy or immunohistochemistry. Recently, there has been signifi cant interest in the process of lymphangiogenesis and the factors that regulate it. To facilitate these studies, we introduce a novel technique for visualization of the immune response during growth factor induced angiogenesis and lymphangiogenesis in the cornea.

本文言語英語
ホスト出版物のタイトルMethods in Molecular Biology
出版社Humana Press Inc.
ページ311-316
ページ数6
DOI
出版ステータス出版済み - 1 1 2016

出版物シリーズ

名前Methods in Molecular Biology
1430
ISSN(印刷版)1064-3745

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

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