The Cys4 zinc finger of bacteriophage T7 primase in sequence-specific single-stranded DNA recognition

Takahiro Kusakabe, Anna V. Hine, Sven G. Hyberts, Charles C. Richardson

研究成果: Contribution to journalArticle査読

53 被引用数 (Scopus)

抄録

Bacteriophage T7 DNA primase recognizes 5'-GTC-3' in single-stranded DNA. The primase contains a single Cys4 zinc-binding motif that is essential for recognition. Biochemical and mutagenic analyses suggest that the Cys4 motif contacts cytosine of 5'-GTC-3' and may also contribute to thymine recognition. Residues His33 and Asp31 are critical for these interactions. Biochemical analysis also reveals that T7 primase selectively binds CTP in the absence of DNA. We propose that bound CTP selects the remaining base G, of 5'-GTC-3', by base pairing. Our deduced mechanism for recognition of ssDNA by Cys4 motifs bears little resemblance to the recognition of trinucleotides of double-stranded DNA by Cys2His2 zinc fingers.

本文言語英語
ページ(範囲)4295-4300
ページ数6
ジャーナルProceedings of the National Academy of Sciences of the United States of America
96
8
DOI
出版ステータス出版済み - 4 13 1999

All Science Journal Classification (ASJC) codes

  • 一般

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