The design of cyclic AMP-recognizing oligopeptides and evaluation of its capability for cyclic AMP recognition using an electrochemical system

Yoshiki Katayama, Yuya Ohuchi, Hideyoshi Higashi, Yoshihisa Kudo, Mizuo Maeda

研究成果: ジャーナルへの寄稿記事

24 引用 (Scopus)

抄録

A novel 17-mer peptide ligand for cyclic AMP was designed using the amino acid sequences of essential subsites in various cyclic AMP-dependent protein kinase (protein kinase A) families. The Au disk electrode, which was modified with the designed 17-mer oligopeptide, responded to cyclic AMP but virtually did not respond to any other cyclic nucleotides using the ion channel sensor mechanism. On the other hand, a scrambled peptide, which had the same amino acid composition as and had an amino acid sequence different from the 17-mer oligopeptide, did not respond to any nucleotides. This indicates that the designed 17-mer peptide actually acted as a selective ligand for cyclic AMP. This ligand-designing strategy using peptide sequences in target-binding proteins may possibly be extended to the design of peptide ligands for other second messengers.

元の言語英語
ページ(範囲)4671-4674
ページ数4
ジャーナルAnalytical Chemistry
72
発行部数19
DOI
出版物ステータス出版済み - 10 1 2000

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Oligopeptides
Cyclic AMP
Peptides
Ligands
Cyclic AMP-Dependent Protein Kinases
Amino Acids
Cyclic Nucleotides
Second Messenger Systems
Ion Channels
Carrier Proteins
Nucleotides
Electrodes
Sensors
Chemical analysis

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

これを引用

The design of cyclic AMP-recognizing oligopeptides and evaluation of its capability for cyclic AMP recognition using an electrochemical system. / Katayama, Yoshiki; Ohuchi, Yuya; Higashi, Hideyoshi; Kudo, Yoshihisa; Maeda, Mizuo.

:: Analytical Chemistry, 巻 72, 番号 19, 01.10.2000, p. 4671-4674.

研究成果: ジャーナルへの寄稿記事

Katayama, Yoshiki ; Ohuchi, Yuya ; Higashi, Hideyoshi ; Kudo, Yoshihisa ; Maeda, Mizuo. / The design of cyclic AMP-recognizing oligopeptides and evaluation of its capability for cyclic AMP recognition using an electrochemical system. :: Analytical Chemistry. 2000 ; 巻 72, 番号 19. pp. 4671-4674.
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