The detection of a breast cancer antigen on MCF-7 cells reactive with the TCR(α) of a specific killer T cell line

Fumiyoshi Okano, Hirofumi Tachibana, Koichi Akiyama, Hiroki Murakami

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

We established several immortalized human T cell lines by cotransfecting primary lymphocytes derived from breast cancer patients with human c-myc and human c-Ha-ras oncogenes. A CD8 positive (CD8+) killer T cell line, FT-8, exhibited in vitro specific cytotoxicity to a human breast cancer cell line, MCF-7. The FT-8 cells suppressed the growth of MCF-7 cells transplanted to athymic mice. The cytotoxic reaction was caused via T cell antigen receptor (TCR) on MCF-7 cells, because monoclonal antibodies against the TCR inhibited the cytotoxicity of FT-8 cells. The TCR(α) cDNA of FT-8 was cloned by using a PCR amplification technique and expressed by a cell-free in vitro translation system. The TCR(α) protein recognized a target antigen of 32 KDa on MCF-7 cells.

元の言語英語
ページ(範囲)195-203
ページ数9
ジャーナルBiotherapy
6
発行部数3
DOI
出版物ステータス出版済み - 9 1 1993

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MCF-7 Cells
T-Cell Antigen Receptor
Breast Neoplasms
T-Lymphocytes
Antigens
Cell Line
ras Genes
Nude Mice
Complementary DNA
Monoclonal Antibodies
Lymphocytes
Polymerase Chain Reaction
Growth
Proteins
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Pharmacology

これを引用

The detection of a breast cancer antigen on MCF-7 cells reactive with the TCR(α) of a specific killer T cell line. / Okano, Fumiyoshi; Tachibana, Hirofumi; Akiyama, Koichi; Murakami, Hiroki.

:: Biotherapy, 巻 6, 番号 3, 01.09.1993, p. 195-203.

研究成果: ジャーナルへの寄稿記事

Okano, Fumiyoshi ; Tachibana, Hirofumi ; Akiyama, Koichi ; Murakami, Hiroki. / The detection of a breast cancer antigen on MCF-7 cells reactive with the TCR(α) of a specific killer T cell line. :: Biotherapy. 1993 ; 巻 6, 番号 3. pp. 195-203.
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