The dual roles of the Golgi transport 1 (GOT1B)

RNA localization to the cortical endoplasmic reticulum and the export of proglutelin and a-globulin from the cortical ER to the Golgi

Masako Fukuda, Yasushi Kawagoe, Takahiro Murakami, Haruhiko Washida, Aya Sugino, Ai Nagamine, Thomas W. Okita, Masahiro Ogawa, Toshihiro Kumamaru

研究成果: ジャーナルへの寄稿記事

8 引用 (Scopus)

抄録

The rice glup2 lines are characterized by their abnormally high levels of endosperm 57 kDa proglutelins and of the luminal chaperone binding protein (BiP), features characteristic of a defect within the endoplasmic reticulum (ER). To elucidate the underlying genetic basis, the glup2 locus was identified by map based cloning. DNA sequencing of the genomes of three glup2 alleles and wild type demonstrated that the underlying genetic basis was mutations in the Golgi transport 1 (GOT1B) coding sequence. This conclusion was further validated by restoration of normal proglutelin levels in a glup2 line complemented by a GOT1B gene. Microscopic analyses indicated the presence of proglutelina-globulin-containing intracisternal granules surrounded by prolamine inclusions within the ER lumen. As assessed by in situ reverse transcriptase polymerase chain reaction (RTPCR) analysis of developing endosperm sections, prolamine and a-globulin RNAs were found to be mis-targeted from their usual sites on the protein body ER to the cisternal ER, the normal sites of proglutelin synthesis. Our results indicate that GLUP2/GOT1B has a dual role during rice endosperm development. It is required for localization of prolamine and a-globulin RNAs to the protein body ER and for efficient export of proglutelin and a-globulin proteins from the ER to the Golgi apparatus.

元の言語英語
ページ(範囲)2380-2391
ページ数12
ジャーナルPlant and Cell Physiology
57
発行部数11
DOI
出版物ステータス出版済み - 11 1 2016

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Globulins
globulins
Endoplasmic Reticulum
endoplasmic reticulum
Phenylpropanolamine
RNA
Endosperm
endosperm
protein bodies
rice
Proteins
Golgi Apparatus
Golgi apparatus
Reverse Transcriptase Polymerase Chain Reaction
DNA Sequence Analysis
binding proteins
Organism Cloning
granules
molecular cloning
Carrier Proteins

All Science Journal Classification (ASJC) codes

  • Physiology
  • Plant Science
  • Cell Biology

これを引用

The dual roles of the Golgi transport 1 (GOT1B) : RNA localization to the cortical endoplasmic reticulum and the export of proglutelin and a-globulin from the cortical ER to the Golgi. / Fukuda, Masako; Kawagoe, Yasushi; Murakami, Takahiro; Washida, Haruhiko; Sugino, Aya; Nagamine, Ai; Okita, Thomas W.; Ogawa, Masahiro; Kumamaru, Toshihiro.

:: Plant and Cell Physiology, 巻 57, 番号 11, 01.11.2016, p. 2380-2391.

研究成果: ジャーナルへの寄稿記事

Fukuda, Masako ; Kawagoe, Yasushi ; Murakami, Takahiro ; Washida, Haruhiko ; Sugino, Aya ; Nagamine, Ai ; Okita, Thomas W. ; Ogawa, Masahiro ; Kumamaru, Toshihiro. / The dual roles of the Golgi transport 1 (GOT1B) : RNA localization to the cortical endoplasmic reticulum and the export of proglutelin and a-globulin from the cortical ER to the Golgi. :: Plant and Cell Physiology. 2016 ; 巻 57, 番号 11. pp. 2380-2391.
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abstract = "The rice glup2 lines are characterized by their abnormally high levels of endosperm 57 kDa proglutelins and of the luminal chaperone binding protein (BiP), features characteristic of a defect within the endoplasmic reticulum (ER). To elucidate the underlying genetic basis, the glup2 locus was identified by map based cloning. DNA sequencing of the genomes of three glup2 alleles and wild type demonstrated that the underlying genetic basis was mutations in the Golgi transport 1 (GOT1B) coding sequence. This conclusion was further validated by restoration of normal proglutelin levels in a glup2 line complemented by a GOT1B gene. Microscopic analyses indicated the presence of proglutelina-globulin-containing intracisternal granules surrounded by prolamine inclusions within the ER lumen. As assessed by in situ reverse transcriptase polymerase chain reaction (RTPCR) analysis of developing endosperm sections, prolamine and a-globulin RNAs were found to be mis-targeted from their usual sites on the protein body ER to the cisternal ER, the normal sites of proglutelin synthesis. Our results indicate that GLUP2/GOT1B has a dual role during rice endosperm development. It is required for localization of prolamine and a-globulin RNAs to the protein body ER and for efficient export of proglutelin and a-globulin proteins from the ER to the Golgi apparatus.",
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