The effect of adrenaline or noradrenaline with or without lidocaine on the contractile response of lipopolysaccharide-treated rat thoracic aortas

Shigeki Fujiwara, Akiko Noguchi, Masanori Tsukamoto, Shinichi Ito, Uno Imaizumi, Yoshinari Morimoto, Kazu Ichi Yoshida, Takeshi Yokoyama

研究成果: ジャーナルへの寄稿記事

1 引用 (Scopus)

抄録

The analgesic effect of a local anesthetic is often insufficient in acutely inflamed tissues, due to the reduced pH and/or vasodilation. Thus, vasoconstrictors (adrenaline or noradrenaline) are often used to contain the anesthetic and prolong the analgesia, although there are minimal data regarding their effects in inflamed tissues. Therefore, we prepared a model of blood vessel inflammation (using lipopolysaccharide), and investigated the contractile effects of adrenaline or noradrenaline with and without lidocaine. Wistar rats’ thoracic aortas were cut into 3-mm-thick rings, which were stretched using a pair of hooks in an organ bath (Krebs-Henseleit solution, 37°C, pH=7.4). After lipopolysaccharide exposure (1 μg/mL), adrenaline or noradrenaline was applied in successive cumulative doses (10-9 to 10-5 M) with or without lidocaine (10-4 M), and the isometric vasocontractions were recorded. The lipopolysaccharide treatment attenuated the vasocontractions that were induced by adrenaline or noradrenaline with lidocaine in a time-dependent manner. Despite its vasodilation properties, lidocaine enhanced the contractile responses that were produced by low concentrations of adrenaline (10-8 to 10-7 M), which indicates that a sufficient analgesic effect depends on the concentration of lidocaine and adrenaline. Therefore, adrenaline should be used when injecting inflamed tissues with lidocaine.

元の言語英語
ページ(範囲)453-457
ページ数5
ジャーナルBiomedical Research (India)
27
発行部数2
出版物ステータス出版済み - 1 1 2016

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Lidocaine
Thoracic Aorta
Epinephrine
Lipopolysaccharides
Rats
Norepinephrine
Tissue
Vasodilation
Analgesics
Hooks
Blood vessels
Vasoconstrictor Agents
Local Anesthetics
Baths
Analgesia
Blood Vessels
Anesthetics
Wistar Rats
Inflammation

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

これを引用

The effect of adrenaline or noradrenaline with or without lidocaine on the contractile response of lipopolysaccharide-treated rat thoracic aortas. / Fujiwara, Shigeki; Noguchi, Akiko; Tsukamoto, Masanori; Ito, Shinichi; Imaizumi, Uno; Morimoto, Yoshinari; Yoshida, Kazu Ichi; Yokoyama, Takeshi.

:: Biomedical Research (India), 巻 27, 番号 2, 01.01.2016, p. 453-457.

研究成果: ジャーナルへの寄稿記事

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