抄録
Synthetic RNAs coding for chicken lysozyme, calf preprochymozin and Xenopus globin were transcribed in vitro using Sp6 RNA polymerase. The effects of capping and adding a poly(A) tail on the stability, movement and translation of these RNAs in Xenopus oocytes was examined.Capping and polyadenylation increased stability of the transcripts, with at least 40% remaining intact 48h after injection into oocytes.Capped poly(A) transcripts moved more rapidly in oocytes than either capped poly(A)+ transcripts or naturally occurring mRNAs.The translational efficiency of most of the synthetic RNAs in oocytes increased with both capping and polyadenylation. The exception was one Xenopus globin transcript which had an unusual 3′ end of 20As and 30Cs, where further polyadenylation decreased translational efficiency.Polyadenylation was essential for detectable expression of the synthetic RNAs in cultured cells, but decreased translation of the synthetic RNAs in vitro.
| 元の言語 | 英語 |
|---|---|
| ページ（範囲） | 7375-7394 |
| ページ数 | 20 |
| ジャーナル | Nucleic acids research |
| 巻 | 13 |
| 発行部数 | 20 |
| DOI | |
| 出版物ステータス | 出版済み - 10 25 1985 |
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All Science Journal Classification (ASJC) codes
- Genetics
これを引用
The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes. / Drummond, Douglas Robert; Armstrong, John; Colman, Alan.
：: Nucleic acids research, 巻 13, 番号 20, 25.10.1985, p. 7375-7394.研究成果: ジャーナルへの寄稿 › 記事
}
TY - JOUR
T1 - The effect of capping and polyadenylation on the stability, movement and translation of synthetic messenger RNAs in Xenopus oocytes
AU - Drummond, Douglas Robert
AU - Armstrong, John
AU - Colman, Alan
PY - 1985/10/25
Y1 - 1985/10/25
N2 - Synthetic RNAs coding for chicken lysozyme, calf preprochymozin and Xenopus globin were transcribed in vitro using Sp6 RNA polymerase. The effects of capping and adding a poly(A) tail on the stability, movement and translation of these RNAs in Xenopus oocytes was examined.Capping and polyadenylation increased stability of the transcripts, with at least 40% remaining intact 48h after injection into oocytes.Capped poly(A) transcripts moved more rapidly in oocytes than either capped poly(A)+ transcripts or naturally occurring mRNAs.The translational efficiency of most of the synthetic RNAs in oocytes increased with both capping and polyadenylation. The exception was one Xenopus globin transcript which had an unusual 3′ end of 20As and 30Cs, where further polyadenylation decreased translational efficiency.Polyadenylation was essential for detectable expression of the synthetic RNAs in cultured cells, but decreased translation of the synthetic RNAs in vitro.
AB - Synthetic RNAs coding for chicken lysozyme, calf preprochymozin and Xenopus globin were transcribed in vitro using Sp6 RNA polymerase. The effects of capping and adding a poly(A) tail on the stability, movement and translation of these RNAs in Xenopus oocytes was examined.Capping and polyadenylation increased stability of the transcripts, with at least 40% remaining intact 48h after injection into oocytes.Capped poly(A) transcripts moved more rapidly in oocytes than either capped poly(A)+ transcripts or naturally occurring mRNAs.The translational efficiency of most of the synthetic RNAs in oocytes increased with both capping and polyadenylation. The exception was one Xenopus globin transcript which had an unusual 3′ end of 20As and 30Cs, where further polyadenylation decreased translational efficiency.Polyadenylation was essential for detectable expression of the synthetic RNAs in cultured cells, but decreased translation of the synthetic RNAs in vitro.
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U2 - 10.1093/nar/13.20.7375
DO - 10.1093/nar/13.20.7375
M3 - Article
VL - 13
SP - 7375
EP - 7394
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 20
ER -