単細胞緑藻 Chlorella vulgaris IAM C-27 株の耐凍性獲得に及ぼすルヌラリン酸の効果

渡邉 雄太, 町田 豪, 山田 直隆, 本城 賢一, 桑野 栄一

研究成果: ジャーナルへの寄稿会談の抜粋

抄録

Many plants acquire freezing tolerance through cold acclimation, a prolonged exposure to low but non-freezing temperatures. We have studied the development of freezing tolerance in plant using Chlorella vulgaris IAM C-27 strain as a convenient model. Chlorella vulgaris IAM C-27 is known to develop its freezing tolerance during the treatment of 3℃ for 24 hours, called hardening. It was demonstrated that exogenous treatment of ABA increased in freezing tolerance in various higher plants. Lunularic acid (LA) was first isolated from the liverwort Lunularia cruciata as a growth-inhibiting and dormancy-inducing factor. It has also been reported that LA is an endogenous growth regulator in the lower plants. We have found that the treatment of LA during hardening at 3℃, 24 hours inhibits the growth of chlorella cells after freezing at -20℃. On the other hand, the treatments of LA at 10℃ accelerate their recovery in growth. To examine the mechanism of developing in freezing tolerance via LA, we researched the hiC (hardening-induced chlorella) gene expressions with LA treatments at 10℃. It was reported that the HIC6 and HIC12 protein were homologous to late embryogenesis abundant (LEA) proteins. The result of Real time RT-PCR suggested that mechanism of the freezing tolerance induction via LA was different from that involved in expression hiC genes.
元の言語Japanese
ページ(範囲)62
ジャーナル植物の生長調節 = Regulation of plant growth & development
42
出版物ステータス出版済み - 10 5 2007

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Chlorella vulgaris
cold tolerance
acids
Chlorella
acid treatment
growth regulators
dormancy
mosses and liverworts
abscisic acid
acclimation
freezing
embryogenesis
proteins
reverse transcriptase polymerase chain reaction
gene expression
temperature
genes

これを引用

単細胞緑藻 Chlorella vulgaris IAM C-27 株の耐凍性獲得に及ぼすルヌラリン酸の効果. / 渡邉雄太; 町田豪; 山田直隆; 本城賢一; 桑野栄一.

:: 植物の生長調節 = Regulation of plant growth & development, 巻 42, 05.10.2007, p. 62.

研究成果: ジャーナルへの寄稿会談の抜粋

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title = "単細胞緑藻 Chlorella vulgaris IAM C-27 株の耐凍性獲得に及ぼすルヌラリン酸の効果",
abstract = "Many plants acquire freezing tolerance through cold acclimation, a prolonged exposure to low but non-freezing temperatures. We have studied the development of freezing tolerance in plant using Chlorella vulgaris IAM C-27 strain as a convenient model. Chlorella vulgaris IAM C-27 is known to develop its freezing tolerance during the treatment of 3℃ for 24 hours, called hardening. It was demonstrated that exogenous treatment of ABA increased in freezing tolerance in various higher plants. Lunularic acid (LA) was first isolated from the liverwort Lunularia cruciata as a growth-inhibiting and dormancy-inducing factor. It has also been reported that LA is an endogenous growth regulator in the lower plants. We have found that the treatment of LA during hardening at 3℃, 24 hours inhibits the growth of chlorella cells after freezing at -20℃. On the other hand, the treatments of LA at 10℃ accelerate their recovery in growth. To examine the mechanism of developing in freezing tolerance via LA, we researched the hiC (hardening-induced chlorella) gene expressions with LA treatments at 10℃. It was reported that the HIC6 and HIC12 protein were homologous to late embryogenesis abundant (LEA) proteins. The result of Real time RT-PCR suggested that mechanism of the freezing tolerance induction via LA was different from that involved in expression hiC genes.",
author = "雄太 渡邉 and 豪 町田 and 直隆 山田 and 賢一 本城 and 栄一 桑野",
year = "2007",
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T1 - 単細胞緑藻 Chlorella vulgaris IAM C-27 株の耐凍性獲得に及ぼすルヌラリン酸の効果

AU - 渡邉, 雄太

AU - 町田, 豪

AU - 山田, 直隆

AU - 本城, 賢一

AU - 桑野, 栄一

PY - 2007/10/5

Y1 - 2007/10/5

N2 - Many plants acquire freezing tolerance through cold acclimation, a prolonged exposure to low but non-freezing temperatures. We have studied the development of freezing tolerance in plant using Chlorella vulgaris IAM C-27 strain as a convenient model. Chlorella vulgaris IAM C-27 is known to develop its freezing tolerance during the treatment of 3℃ for 24 hours, called hardening. It was demonstrated that exogenous treatment of ABA increased in freezing tolerance in various higher plants. Lunularic acid (LA) was first isolated from the liverwort Lunularia cruciata as a growth-inhibiting and dormancy-inducing factor. It has also been reported that LA is an endogenous growth regulator in the lower plants. We have found that the treatment of LA during hardening at 3℃, 24 hours inhibits the growth of chlorella cells after freezing at -20℃. On the other hand, the treatments of LA at 10℃ accelerate their recovery in growth. To examine the mechanism of developing in freezing tolerance via LA, we researched the hiC (hardening-induced chlorella) gene expressions with LA treatments at 10℃. It was reported that the HIC6 and HIC12 protein were homologous to late embryogenesis abundant (LEA) proteins. The result of Real time RT-PCR suggested that mechanism of the freezing tolerance induction via LA was different from that involved in expression hiC genes.

AB - Many plants acquire freezing tolerance through cold acclimation, a prolonged exposure to low but non-freezing temperatures. We have studied the development of freezing tolerance in plant using Chlorella vulgaris IAM C-27 strain as a convenient model. Chlorella vulgaris IAM C-27 is known to develop its freezing tolerance during the treatment of 3℃ for 24 hours, called hardening. It was demonstrated that exogenous treatment of ABA increased in freezing tolerance in various higher plants. Lunularic acid (LA) was first isolated from the liverwort Lunularia cruciata as a growth-inhibiting and dormancy-inducing factor. It has also been reported that LA is an endogenous growth regulator in the lower plants. We have found that the treatment of LA during hardening at 3℃, 24 hours inhibits the growth of chlorella cells after freezing at -20℃. On the other hand, the treatments of LA at 10℃ accelerate their recovery in growth. To examine the mechanism of developing in freezing tolerance via LA, we researched the hiC (hardening-induced chlorella) gene expressions with LA treatments at 10℃. It was reported that the HIC6 and HIC12 protein were homologous to late embryogenesis abundant (LEA) proteins. The result of Real time RT-PCR suggested that mechanism of the freezing tolerance induction via LA was different from that involved in expression hiC genes.

UR - http://ci.nii.ac.jp/naid/110006474742

M3 - 会談の抜粋

VL - 42

SP - 62

JO - 植物の生長調節 = Regulation of plant growth & development

JF - 植物の生長調節 = Regulation of plant growth & development

SN - 1346-5406

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