The effects of flavoxate hydrochloride on voltage-dependent L-type Ca 2+ currents in human urinary bladder

Toshihisa Tomoda, Manami Aishima, Naruaki Takano, Toshiaki Nakano, Narihito Seki, Yoshikazu Yonemitsu, Katsuo Sueishi, Seiji Naito, Yushi Ito, Noriyoshi Teramoto

研究成果: ジャーナルへの寄稿記事

13 引用 (Scopus)

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The effects of flavoxate hydrochloride (Bladderon®, piperidinoethyl-3-methylflavone-8-carboxylate; hereafter referred as flavoxate) on voltage-dependent nifedipine-sensitive inward Ba 2+ currents in human detrusor myocytes were investigated using a conventional whole-cell patch-clamp. Tension measurement was also performed to study the effects of flavoxate on K +-induced contraction in human urinary bladder. Flavoxate caused a concentration-dependent reduction of the K +-induced contraction of human urinary bladder. In human detrusor myocytes, flavoxate inhibited the peak amplitude of voltage-dependent nifedipine-sensitive inward Ba 2+ currents in a voltage- and concentration-dependent manner (K i = 10 μM), and shifted the steady-state inactivation curve of Ba 2+ currents to the left at a holding potential of -90mV. Immunohistochemical studies indicated the presence of the α 1c subunit protein, which is a constituent of human L-type Ca 2+ channels (Ca v1-2), in the bundles of human detrusor smooth muscle. These results suggest that flavoxate caused muscle relaxation through the inhibition of L-type Ca 2+ channels in human detrusor.

元の言語英語
ページ(範囲)25-32
ページ数8
ジャーナルBritish Journal of Pharmacology
146
発行部数1
DOI
出版物ステータス出版済み - 12 1 2005

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All Science Journal Classification (ASJC) codes

  • Pharmacology

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