The expression of costimulatory molecules CD80 and CD86 in human carcinoma cell lines: Its regulation by interferon γ and interleukin-10

Jian Li, Yi Yang, Hiroshi Inoue, Masaki Mori, Tsuyoshi Akiyoshi

研究成果: Contribution to journalArticle査読

34 被引用数 (Scopus)

抄録

The costimulatory signal through CD80 or CD86 to its counterreceptor CD28 or CTLA-4 on T cells has been shown to play an important role in the induction of T-cell mediated immunity against tumors. In the present study, we examined the expression of CD80 and CD86 in the cell lines derived from human gastric, esophageal and colorectal carcinomas at the mRNA level, by means of a reverse transcriptase/polymerase chain reaction analysis and, for their surface expression, using a flow-cytometric analysis with monoclonal antibodies (mAb). The expression of mRNA for CD80 or CD86 was detected in all 18 cell lines tested, except for CD86 on one cell line. The cells from 13 (72%) or 12 (67%) of these cell lines expressed the surface CD80 or CD86 molecule, detected with the respective mAb. The surface expression of CD80 or CD86 was increased in four to five of the six cell lines tested after a culture with interferon γ (IFNγ). In addition, the up-regulation of CD80 or CD86 expression by IFNγ was inhibited by interleukin-10 (IL-10). These results indicated that, in the cell lines derived from human gastrointestinal carcinoma, both the costimulatory molecules CD80 and CD86 were detectable in the majority of the cell lines examined at the mRNA and protein levels, and could be regulated with the cytokine IFNγ or IL-10.

本文言語英語
ページ(範囲)213-219
ページ数7
ジャーナルCancer Immunology Immunotherapy
43
4
DOI
出版ステータス出版済み - 12 1 1996

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Immunology
  • Oncology
  • Cancer Research

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