The expression of SF-1/Ad4BP is related to the process of luteinization in the marmoset (Callithrix jacchus) ovary

Uwe Wehrenberg, Christine Wulff, Bettina Husen, Ken Ichirou Morohashi, Gabriele M. Rune

研究成果: Contribution to journalArticle査読

10 被引用数 (Scopus)

抄録

Expression of the nuclear receptor, steroidogenic factor-1 (SF-1/Ad4BP), was studied in a primate (marmoset) ovary using immunohistochemical, RT-PCR, and immunoblot techniques. The periovulatory phase was compared with the luteal phase. With PCR we found a marmoset homolog of SF-1/Ad4BP to be expressed in ovarian and other steroidogenic tissues. Characteristically, the periovulatory ovaries consisted of growing (nonluteinized) small follicles together with large luteinizing follicles and many corpora lutea accessoria (Clas), which had developed from atretic large follicles. During the luteal phase, true corpora lutea (Cls) were additionally found. In general, we found that small follicles were devoid of any immunoreactivity of SF-1/Ad4BP. In large follicles, the luteinizing theca and granulosa cells express SF-1/Ad4BP. All luteal cells of Clas showed a nuclear staining in both ovary types. In Cls, only a few luteal cells were positive. Large follicles of different sizes showed no differences in expression level, as evidenced by immunoblot analysis. Our results indicate that SF-1/Ad4BP participates in the activation of gene transcription during the onset of luteinization and that Clas are essential for ovarian luteal function.

本文言語英語
ページ(範囲)345-350
ページ数6
ジャーナルHistochemistry and Cell Biology
107
4
DOI
出版ステータス出版済み - 1997

All Science Journal Classification (ASJC) codes

  • 組織学
  • 分子生物学
  • 医療検査技術
  • 細胞生物学

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