The F-box protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for c-Myc-regulated transcription

Natalie Von Der Lehr, Sara Johansson, Siqin Wu, Fuad Bahram, Alina Castell, Cihan Cetinkaya, Per Hydbring, Ingrid Weidung, Keiko Nakayama, Keiichi I. Nakayama, Ola Söderberg, Tom K. Kerppola, Lars Gunnar Larsson

研究成果: ジャーナルへの寄稿記事

354 引用 (Scopus)

抄録

The transcription regulatory oncoprotein c-Myc controls genes involved in cell growth, apoptosis, and oncogenesis. c-Myc is turned over very quickly through the ubiquitin/proteasome pathway. The proteins involved in this process are still unknown. We have found that Skp2 interacts with c-Myc and participates in its ubiquitylation and degradation. The interaction between Skp2 and c-Myc occurs during the G1 to S phase transition of the cell cycle in normal lymphocytes. Surprisingly, Skp2 enhances c-Myc-induced S phase transition and activates c-Myc target genes in a Myc-dependent manner. Further, Myc-induced transcription was shown to be Skp2 dependent, suggesting interdependence between c-Myc and Skp2 in activation of transcription. Moreover, Myc-dependent association of Skp2, ubiquitylated proteins, and subunits of the proteasome to a c-Myc target promoter was demonstrated in vivo. The results suggest that Skp2 is a transcriptional cofactor for c-Myc and indicates a close relationship between transcription activation and transcription factor ubiquitination.

元の言語英語
ページ(範囲)1189-1200
ページ数12
ジャーナルMolecular Cell
11
発行部数5
DOI
出版物ステータス出版済み - 5 1 2003

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F-Box Proteins
myc Genes
Ubiquitination
Phase Transition
Proteasome Endopeptidase Complex
S Phase
Transcriptional Activation
S-Phase Kinase-Associated Proteins
Oncogene Proteins
Protein Subunits
Ubiquitin
Cell Cycle
Carcinogenesis
Transcription Factors
Lymphocytes
Apoptosis
Growth
Proteins

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Cell Biology

これを引用

Von Der Lehr, N., Johansson, S., Wu, S., Bahram, F., Castell, A., Cetinkaya, C., ... Larsson, L. G. (2003). The F-box protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for c-Myc-regulated transcription. Molecular Cell, 11(5), 1189-1200. https://doi.org/10.1016/S1097-2765(03)00193-X

The F-box protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for c-Myc-regulated transcription. / Von Der Lehr, Natalie; Johansson, Sara; Wu, Siqin; Bahram, Fuad; Castell, Alina; Cetinkaya, Cihan; Hydbring, Per; Weidung, Ingrid; Nakayama, Keiko; Nakayama, Keiichi I.; Söderberg, Ola; Kerppola, Tom K.; Larsson, Lars Gunnar.

:: Molecular Cell, 巻 11, 番号 5, 01.05.2003, p. 1189-1200.

研究成果: ジャーナルへの寄稿記事

Von Der Lehr, N, Johansson, S, Wu, S, Bahram, F, Castell, A, Cetinkaya, C, Hydbring, P, Weidung, I, Nakayama, K, Nakayama, KI, Söderberg, O, Kerppola, TK & Larsson, LG 2003, 'The F-box protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for c-Myc-regulated transcription', Molecular Cell, 巻. 11, 番号 5, pp. 1189-1200. https://doi.org/10.1016/S1097-2765(03)00193-X
Von Der Lehr, Natalie ; Johansson, Sara ; Wu, Siqin ; Bahram, Fuad ; Castell, Alina ; Cetinkaya, Cihan ; Hydbring, Per ; Weidung, Ingrid ; Nakayama, Keiko ; Nakayama, Keiichi I. ; Söderberg, Ola ; Kerppola, Tom K. ; Larsson, Lars Gunnar. / The F-box protein Skp2 participates in c-Myc proteosomal degradation and acts as a cofactor for c-Myc-regulated transcription. :: Molecular Cell. 2003 ; 巻 11, 番号 5. pp. 1189-1200.
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abstract = "The transcription regulatory oncoprotein c-Myc controls genes involved in cell growth, apoptosis, and oncogenesis. c-Myc is turned over very quickly through the ubiquitin/proteasome pathway. The proteins involved in this process are still unknown. We have found that Skp2 interacts with c-Myc and participates in its ubiquitylation and degradation. The interaction between Skp2 and c-Myc occurs during the G1 to S phase transition of the cell cycle in normal lymphocytes. Surprisingly, Skp2 enhances c-Myc-induced S phase transition and activates c-Myc target genes in a Myc-dependent manner. Further, Myc-induced transcription was shown to be Skp2 dependent, suggesting interdependence between c-Myc and Skp2 in activation of transcription. Moreover, Myc-dependent association of Skp2, ubiquitylated proteins, and subunits of the proteasome to a c-Myc target promoter was demonstrated in vivo. The results suggest that Skp2 is a transcriptional cofactor for c-Myc and indicates a close relationship between transcription activation and transcription factor ubiquitination.",
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AU - Castell, Alina

AU - Cetinkaya, Cihan

AU - Hydbring, Per

AU - Weidung, Ingrid

AU - Nakayama, Keiko

AU - Nakayama, Keiichi I.

AU - Söderberg, Ola

AU - Kerppola, Tom K.

AU - Larsson, Lars Gunnar

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