TY - JOUR
T1 - The FHIT gene, spanning the chromosome 3p14.2 fragile site and renal carcinoma-associated t(3;8) breakpoint, is abnormal in digestive tract cancers
AU - Ohta, Masataka
AU - Inoue, Hiroshi
AU - Cotticelli, Maria Grazia
AU - Kastury, Kumar
AU - Baffa, Raffaele
AU - Palazzo, Juan
AU - Siprashvili, Zurab
AU - Mori, Masaki
AU - McCue, Peter
AU - Druck, Teresa
AU - Croce, Carlo M.
AU - Huebner, Kay
N1 - Funding Information:
Correspondence should be addressed to K. H. This work was supported by United States Public Health Service grants CA51083, CA39860, and CA21124 and a gift from R. R. M. Carpenter III and M. K. Carpenter; National Cancer Institute Cancer Center grant CA56336 supported the Kimmel Cancer Center shared research facilities that expedited the study. We thank C. Williams for collection of tissues and Drs. L. Barnes and P. Garrison for helpful discussions of diadenosine tetraphosphate hydrolases. We also thank M. Shimizu for expert technical assistance and A. Mathis for preparation of the manuscript.
PY - 1996/2/23
Y1 - 1996/2/23
N2 - A 200-300 kb region of chromosome 3p14.2, including the fragile site locus FRA3B, is homozygously deleted in multiple tumor-derived cell lines. Exon amplification from cosmids covering this deleted region allowed identification of the human FHIT gene, a member of the histidine triad gene family, which encodes a protein with 69% similarity to an S. pombe enzyme, diadenosine 5', 5'' P1, P4-tetraphosphate asymmetrical hydrolase. The FHIT locus is composed of ten exons distributed over at least 500 kb, with three 5' untranslated exons centromeric to the renal carcinoma associated 3p14.2 breakpoint, the remaining exons telomeric to this translocation breakpoint, and exon 5 within the homozygously deleted fragile region. Aberrant transcripts of the FHIT locus were found in ~50% of esophageal, stomach, and colon carcinomas.
AB - A 200-300 kb region of chromosome 3p14.2, including the fragile site locus FRA3B, is homozygously deleted in multiple tumor-derived cell lines. Exon amplification from cosmids covering this deleted region allowed identification of the human FHIT gene, a member of the histidine triad gene family, which encodes a protein with 69% similarity to an S. pombe enzyme, diadenosine 5', 5'' P1, P4-tetraphosphate asymmetrical hydrolase. The FHIT locus is composed of ten exons distributed over at least 500 kb, with three 5' untranslated exons centromeric to the renal carcinoma associated 3p14.2 breakpoint, the remaining exons telomeric to this translocation breakpoint, and exon 5 within the homozygously deleted fragile region. Aberrant transcripts of the FHIT locus were found in ~50% of esophageal, stomach, and colon carcinomas.
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U2 - 10.1016/S0092-8674(00)81034-X
DO - 10.1016/S0092-8674(00)81034-X
M3 - Article
C2 - 8598045
AN - SCOPUS:13344279424
SN - 0092-8674
VL - 84
SP - 587
EP - 597
JO - Cell
JF - Cell
IS - 4
ER -
