The glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of DHA-rich glycerolipids in Aurantiochytrium limacinum F26-b

Eri Nutahara, Eriko Abe, Shinya Uno, Yohei Ishibashi, Takashi Watanabe, Masahiro Hayashi, Nozomu Okino, Makoto Ito

研究成果: ジャーナルへの寄稿記事

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抄録

Thraustochytrids possess docosahexaenoic acid (DHA, 22:6n-3) as acyl chain(s) of triacylglycerol (TG) and phosphatidylcholine (PC), some of which contain multiple DHAs. However, little is known about how these DHA-rich glycerolipids are produced in thraustochytrids. In this study, we identified PLAT2 in Aurantiochytrium limacinum F26-b as a glycerol-3-phosphate (G3P) acyltransferase (GPAT) by heterologous expression of the gene in budding yeast. Subsequently, we found that GPAT activity was reduced by disruption of the PLAT2 gene in A. limacinum, resulting in a decrease in DHA-containing lysophosphatidic acid (LPA 22:6). Conversely, overexpression of PLAT2 increased both GPAT activity and LPA 22:6. These results indicate that PLAT2 is a GPAT that transfers DHA to G3P in vivo as well as in vitro. Overexpression of the PLAT2 gene increased the production of a two DHA-containing diacylglycerol (DG 44:12), followed by an increase in the three DHA-containing TG (TG 66:18), two-DHA-containing TG (TG 60:12), and two DHA-containing PC (PC 44:12). However, overexpression of PLAT2 did not increase DHA-free DG (DG32:0), which was preferentially converted to three 16:0-containing TG (TG 48:0) but not two 16:0-contain-ing PC (PC 32:0). Collectively, we revealed that DHA-rich glycerolipids are produced from a precursor, LPA 22:6, which is generated by incorporating DHA to G3P by PLAT2 in the A. limacinum.

元の言語英語
記事番号e0211164
ジャーナルPloS one
14
発行部数1
DOI
出版物ステータス出版済み - 1 1 2019

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glycerol-3-phosphate acyltransferase
Acyltransferases
Triglycerides
triacylglycerols
Phosphatidylcholines
phosphatidylcholines
acyltransferases
Genes
glycerol
phosphates
gene overexpression
Saccharomycetales
Docosahexaenoic Acids
Diglycerides
diacylglycerols
gene targeting
alpha-glycerophosphoric acid
docosahexaenoic acid
Yeast
yeasts

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

これを引用

The glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of DHA-rich glycerolipids in Aurantiochytrium limacinum F26-b. / Nutahara, Eri; Abe, Eriko; Uno, Shinya; Ishibashi, Yohei; Watanabe, Takashi; Hayashi, Masahiro; Okino, Nozomu; Ito, Makoto.

:: PloS one, 巻 14, 番号 1, e0211164, 01.01.2019.

研究成果: ジャーナルへの寄稿記事

Nutahara, Eri ; Abe, Eriko ; Uno, Shinya ; Ishibashi, Yohei ; Watanabe, Takashi ; Hayashi, Masahiro ; Okino, Nozomu ; Ito, Makoto. / The glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of DHA-rich glycerolipids in Aurantiochytrium limacinum F26-b. :: PloS one. 2019 ; 巻 14, 番号 1.
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abstract = "Thraustochytrids possess docosahexaenoic acid (DHA, 22:6n-3) as acyl chain(s) of triacylglycerol (TG) and phosphatidylcholine (PC), some of which contain multiple DHAs. However, little is known about how these DHA-rich glycerolipids are produced in thraustochytrids. In this study, we identified PLAT2 in Aurantiochytrium limacinum F26-b as a glycerol-3-phosphate (G3P) acyltransferase (GPAT) by heterologous expression of the gene in budding yeast. Subsequently, we found that GPAT activity was reduced by disruption of the PLAT2 gene in A. limacinum, resulting in a decrease in DHA-containing lysophosphatidic acid (LPA 22:6). Conversely, overexpression of PLAT2 increased both GPAT activity and LPA 22:6. These results indicate that PLAT2 is a GPAT that transfers DHA to G3P in vivo as well as in vitro. Overexpression of the PLAT2 gene increased the production of a two DHA-containing diacylglycerol (DG 44:12), followed by an increase in the three DHA-containing TG (TG 66:18), two-DHA-containing TG (TG 60:12), and two DHA-containing PC (PC 44:12). However, overexpression of PLAT2 did not increase DHA-free DG (DG32:0), which was preferentially converted to three 16:0-containing TG (TG 48:0) but not two 16:0-contain-ing PC (PC 32:0). Collectively, we revealed that DHA-rich glycerolipids are produced from a precursor, LPA 22:6, which is generated by incorporating DHA to G3P by PLAT2 in the A. limacinum.",
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T1 - The glycerol-3-phosphate acyltransferase PLAT2 functions in the generation of DHA-rich glycerolipids in Aurantiochytrium limacinum F26-b

AU - Nutahara, Eri

AU - Abe, Eriko

AU - Uno, Shinya

AU - Ishibashi, Yohei

AU - Watanabe, Takashi

AU - Hayashi, Masahiro

AU - Okino, Nozomu

AU - Ito, Makoto

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