The growth-promoting activity of egg white proteins in the C2C12 myoblast cell line

Wataru Mizunoya, Ayumi Tashima, Yusuke Sato, Ryuichi Tatsumi, Yoshihide Ikeuchi

研究成果: ジャーナルへの寄稿学術誌査読

16 被引用数 (Scopus)


In this study, we examined the effects of several egg white proteins (ovalbumin, ovomucoid, ovotransferrin and lysozyme) on proliferation and myotube growth in C2C12 murine myoblast cells. Cell proliferation was measured using a water-soluble tetrazolium salt (WST-8)-based assay and then validated using Giemsa staining. Significant proliferative activities of C2C12 cells were observed in response to the addition of 10(-5) -10(-4) mol/L ovalbumin or ovomucoid. Ovotransferrin decreased C2C12 cell proliferation and lysozyme showed no significant effects on the proliferation of C2C12 cells. In contrast, the proliferative effects of ovalbumin and ovomucoid were not observed in 3T3-L1 murine preadipocyte cells. We also measured the effects of ovalbumin and ovomucoid on C2C12 myotube diameters by using histological analysis. In comparison to control cells, myotube diameters were significantly increased in cells cultured in 10(-6) -10(-4) mol/L ovalbumin or ovomucoid, suggesting that ovalbumin and ovomucoid stimulate the growth of myotubes. Thus, our results clearly demonstrated that ovalbumin or ovomucoid stimulated the proliferation of myoblasts and growth of myotubes.

ジャーナルAnimal science journal = Nihon chikusan Gakkaihō
出版ステータス出版済み - 2月 1 2015

!!!All Science Journal Classification (ASJC) codes

  • 農業および生物科学(全般)


「The growth-promoting activity of egg white proteins in the C2C12 myoblast cell line」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。