The heterochromatin protein Swi6/HP1 activates replication origins at the pericentromeric region and silent mating-type locus

Makoto T. Hayashi, Tatsuro S. Takahashi, Takuro Nakagawa, Jun Ichi Nakayama, Hisao Masukata

研究成果: Contribution to journalArticle査読

116 被引用数 (Scopus)

抄録

Heterochromatin is a structurally compacted region of chromosomes in which transcription and recombination are inactivated. DNA replication is temporally regulated in heterochromatin, but the molecular mechanism for regulation has not been elucidated. Among heterochromatin loci in Schizosaccharomyces pombe, the pericentromeric region and the silent mating-type (mat) locus replicate in early S phase, whereas the sub-telomeric region does not, suggesting complex mechanisms for regulation of replication in heterochromatic regions. Here, we show that Swi6, an S. pombe counterpart of heterochromatin protein 1 (HP1), is required for early replication of the pericentromeric region and the mat locus. Origin-loading of Sld3, which depends on Dfp1/Dbf4-dependent kinase Cdc7 (DDK), is stimulated by Swi6. An HP1-binding motif within Dfp1 is required for interaction with Swi6 in vitro and for early replication of the pericentromeric region and mat locus. Tethering of Dfp1 to the pericentromeric region and mat locus in swi6-deficient cells restores early replication of these loci. Our results show that a heterochromatic protein positively regulates initiation of replication in silenced chromatin by interacting with an essential kinase.

本文言語英語
ページ(範囲)357-362
ページ数6
ジャーナルNature Cell Biology
11
3
DOI
出版ステータス出版済み - 2009

All Science Journal Classification (ASJC) codes

  • Cell Biology

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