The induction of chromosome condensation in tsBN2, a temperature-sensitive mutant of BHK21, inhibited by the calmodulin antagonist, W-7

Takeharu Nishimoto, Kozo Ajiro, Masato Hirata, Katsumi Yamashita, Mutsuo Sekiguchi

研究成果: ジャーナルへの寄稿記事

11 引用 (Scopus)

抄録

The induction of premature chromosome condensation (PCC) in tsBN2 cells, a temperature-sensitive (ts) mutant of BHK21/13 which shows PCC at the non-permissive temperature, was almost completely inhibited by 40 μM W-7, an antagonist of calmodulin. The mitotic phosphorylation of histone H1 and H3 was also inhibited by W-7, W-5, a chlorine-deficient analogue of W-7 and which interacts weakly with calmodulin, did not inhibit the induction of PCC, even at a dose of 80 μM. The content of calmodulin in tsBN2 cells was increased by a temperature shift to 40.5°C. All these results suggested that calmodulin is required for the chromosome condensation.

元の言語英語
ページ(範囲)351-358
ページ数8
ジャーナルExperimental Cell Research
156
発行部数2
DOI
出版物ステータス出版済み - 1 1 1985

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Calmodulin
Chromosomes
Temperature
Histones
Chlorine
Phosphorylation
W 7

All Science Journal Classification (ASJC) codes

  • Cell Biology

これを引用

The induction of chromosome condensation in tsBN2, a temperature-sensitive mutant of BHK21, inhibited by the calmodulin antagonist, W-7. / Nishimoto, Takeharu; Ajiro, Kozo; Hirata, Masato; Yamashita, Katsumi; Sekiguchi, Mutsuo.

:: Experimental Cell Research, 巻 156, 番号 2, 01.01.1985, p. 351-358.

研究成果: ジャーナルへの寄稿記事

Nishimoto, Takeharu ; Ajiro, Kozo ; Hirata, Masato ; Yamashita, Katsumi ; Sekiguchi, Mutsuo. / The induction of chromosome condensation in tsBN2, a temperature-sensitive mutant of BHK21, inhibited by the calmodulin antagonist, W-7. :: Experimental Cell Research. 1985 ; 巻 156, 番号 2. pp. 351-358.
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abstract = "The induction of premature chromosome condensation (PCC) in tsBN2 cells, a temperature-sensitive (ts) mutant of BHK21/13 which shows PCC at the non-permissive temperature, was almost completely inhibited by 40 μM W-7, an antagonist of calmodulin. The mitotic phosphorylation of histone H1 and H3 was also inhibited by W-7, W-5, a chlorine-deficient analogue of W-7 and which interacts weakly with calmodulin, did not inhibit the induction of PCC, even at a dose of 80 μM. The content of calmodulin in tsBN2 cells was increased by a temperature shift to 40.5°C. All these results suggested that calmodulin is required for the chromosome condensation.",
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