The intercellular distribution of glycine decarboxylase in leaves of cassava in relation to the photosynthetic mode and leaf anatomy

Osamu Ueno, Sakae Agarie

研究成果: ジャーナルへの寄稿学術誌査読

8 被引用数 (Scopus)

抄録

There have been conflicting reports on the photosynthetic mode of cassava (Manihot esculenta Crantz). It has been suggested that cassava is a C3-C4 intermediate plant and, alternatively, that it is a C3 plant. Glycine decarboxylase (GDC), a photorespiratory enzyme, is known to exhibit different patterns of intercellular distribution between C3-C4 intermediate and C3 leaves. In order to elucidate the photosynthetic mode of cassava, we investigated the localization of GDC in the leaves of three cultivars by immunogold electron microscopy and the anatomical structures of the leaves. The leaves of cassava are essentially hypostomatous, and the photosynthetic tissues consist of palisade mesophyll cells (PMCs), spongy mesophyll cells (SMCs) and bundle sheath cells (BSCs). Although the BSCs include centrifugally located chloroplasts, they show no increase in mitochondrial frequency. Labeling specific for GDC was found on all mitochondria of all three types of cell. However, the density of labeling in the PMGs was always higher than in the SMCs and BSCs. In leaves that developed under a water deficit, the difference in labeling density became even clearer. These data suggest that at least the cultivars of cassava examined here are not C3-C4 intermediates and should be regarded as C3 plants. However, the intercellular differences in the level of accumulation of GDC seem to merit further investigation with respect to an internal gradient of photorespiratory capacity in the leaves.

本文言語英語
ページ(範囲)268-278
ページ数11
ジャーナルJapanese Journal of Crop Science
66
2
DOI
出版ステータス出版済み - 6月 1997

!!!All Science Journal Classification (ASJC) codes

  • 食品科学
  • 農業および作物学
  • 遺伝学

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