The kinesin-14 Klp2 organizes microtubules into parallel bundles by an ATP-dependent sorting mechanism

Marcus Braun, Douglas R. Drummond, Robert A. Cross, Andrew D. McAinsh

研究成果: ジャーナルへの寄稿学術誌査読

86 被引用数 (Scopus)

抄録

The dynamic organization of microtubules into parallel arrays allows interphase cells to set up multi-lane highways for intracellular transport and M-phase cells to build the mitotic and meiotic spindles. Here we show that a minimally reconstituted system composed of Klp2, a kinesin-14 from the fission yeast Schizosaccharomyces pombe, together with microtubules assembled from purified S. pombe tubulin, autonomously assembles bundles of parallel microtubules. Bundles form by an ATP-dependent sorting mechanism that requires the full-length Klp2 motor. By this mechanism, antiparallel-overlapped microtubules slide over one another until they dissociate from the bundles, whereas parallel-overlapped microtubules are selectively trapped by an energy-dissipating force-balance mechanism. Klp2-driven microtubule sorting provides a robust pathway for the organization of microtubules into parallel arrays. In vivo evidence indicates that Klp2 is required for the proper organization of S. pombe interphase microtubules into bipolar arrays of parallel-overlapped microtubules, suggesting that kinesin-14-dependent microtubule sorting may have wide biological importance.

本文言語英語
ページ(範囲)724-730
ページ数7
ジャーナルNature Cell Biology
11
6
DOI
出版ステータス出版済み - 2009
外部発表はい

!!!All Science Journal Classification (ASJC) codes

  • 細胞生物学

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