The linkage of teleost skin keratan sulfate to protein

Makoto Ito, Tatsuya Yamagata

研究成果: ジャーナルへの寄稿記事

2 引用 (Scopus)

抄録

The linkage of teleost skin keratan sulfate to protein was investigated. Afeter its exhaustive digestion with pronase, peptidokeratan sulfate was obtained with aspartic acid as the predominant amino acid. The N-terminal of the amino acid residues of the preparation was dansylated, and the carbohydrate-peptide linkage fragment was isolated, with the aid of fluorescence, by sequential digestion with Flavobacterium endo-β-galactosidase, β-galactosidase, β-N-acetylhexosaminidase and endo-β-N-acetylglucosaminidase D, followed by Bio-Gel p-4 column chromatography. The structure of the dansylated fragment thus obtained was identified dansylated asparaginyl N-acetyl-D-glucosamine. Treatment of the dansylated keratan sulfate peptide with almond glycopeptidase, which specially cleaves thet asparaginyl N-acetyl-D-glucosamine linkage in the glycoproteins, also showed asparaginyl N-aceytyl-D-glucosamine linkage to be in the core region of this keratan sulfate. We conclude that teleost skin keratan sulfate is bound to protein via an N-glycosyl linkage between N-acetyl-D-glucosamine and asparagine. The keratan sulfate core apparently consist of trimannosyl-N,N′-diacetylchitobiose units, considering the specificity of endo-β-N-acetylglucosaminidase D.

元の言語英語
ページ(範囲)381-387
ページ数7
ジャーナルBBA - General Subjects
801
発行部数3
DOI
出版物ステータス出版済み - 10 16 1984
外部発表Yes

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Keratan Sulfate
Skin
Acetylglucosamine
Galactosidases
Acetylglucosaminidase
Proteins
Digestion
Carbohydrate Conformation
Peptide-N4-(N-acetyl-beta-glucosaminyl) Asparagine Amidase
Flavobacterium
Amino Acids
Pronase
Peptides
Column chromatography
Peptide Fragments
Asparagine
Glucosamine
Aspartic Acid
Sulfates
Chromatography

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

これを引用

The linkage of teleost skin keratan sulfate to protein. / Ito, Makoto; Yamagata, Tatsuya.

:: BBA - General Subjects, 巻 801, 番号 3, 16.10.1984, p. 381-387.

研究成果: ジャーナルへの寄稿記事

Ito, Makoto ; Yamagata, Tatsuya. / The linkage of teleost skin keratan sulfate to protein. :: BBA - General Subjects. 1984 ; 巻 801, 番号 3. pp. 381-387.
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