The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking

Shigemi Sasawatari, Mariko Yoshizaki, Choji Taya, Aya Tazawa, Kaori Furuyama-Tanaka, Hiromichi Yonekawa, Taeko Dohi, Andrew P. Makrigiannis, Takehiko Sasazuki, Kayo Inaba, Noriko Toyama-Sorimachi

研究成果: ジャーナルへの寄稿記事

25 引用 (Scopus)

抄録

Neutrophils rapidly undergo polarization and directional movement to infiltrate the sites of infection and inflammation. Here, we show that an inhibitory MHC I receptor, Ly49Q, was crucial for the swift polarization of and tissue infiltration by neutrophils. During the steady state, Ly49Q inhibited neutrophil adhesion by preventing focal-complex formation, likely by inhibiting Src and PI3 kinases. However, in the presence of inflammatory stimuli, Ly49Q mediated rapid neutrophil polarization and tissue infiltration in an ITIM-domain-dependent manner. These opposite functions appeared to be mediated by distinct use of effector phosphatase SHP-1 and SHP-2. Ly49Q-dependent polarization and migration were affected by Ly49Q regulation of membrane raft functions. We propose that Ly49Q is pivotal in switching neutrophils to their polarized morphology and rapid migration upon inflammation, through its spatiotemporal regulation of membrane rafts and raft-associated signaling molecules.

元の言語英語
ページ(範囲)200-213
ページ数14
ジャーナルImmunity
32
発行部数2
DOI
出版物ステータス出版済み - 2 26 2010
外部発表Yes

Fingerprint

Neutrophils
Non-Receptor Type 6 Protein Tyrosine Phosphatase
Inflammation
Focal Adhesions
Membranes
src-Family Kinases
Neutrophil Infiltration
Phosphatidylinositol 3-Kinases
Infection

All Science Journal Classification (ASJC) codes

  • Immunology and Allergy
  • Infectious Diseases
  • Immunology

これを引用

Sasawatari, S., Yoshizaki, M., Taya, C., Tazawa, A., Furuyama-Tanaka, K., Yonekawa, H., ... Toyama-Sorimachi, N. (2010). The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking. Immunity, 32(2), 200-213. https://doi.org/10.1016/j.immuni.2010.01.012

The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking. / Sasawatari, Shigemi; Yoshizaki, Mariko; Taya, Choji; Tazawa, Aya; Furuyama-Tanaka, Kaori; Yonekawa, Hiromichi; Dohi, Taeko; Makrigiannis, Andrew P.; Sasazuki, Takehiko; Inaba, Kayo; Toyama-Sorimachi, Noriko.

:: Immunity, 巻 32, 番号 2, 26.02.2010, p. 200-213.

研究成果: ジャーナルへの寄稿記事

Sasawatari, S, Yoshizaki, M, Taya, C, Tazawa, A, Furuyama-Tanaka, K, Yonekawa, H, Dohi, T, Makrigiannis, AP, Sasazuki, T, Inaba, K & Toyama-Sorimachi, N 2010, 'The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking', Immunity, 巻. 32, 番号 2, pp. 200-213. https://doi.org/10.1016/j.immuni.2010.01.012
Sasawatari S, Yoshizaki M, Taya C, Tazawa A, Furuyama-Tanaka K, Yonekawa H その他. The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking. Immunity. 2010 2 26;32(2):200-213. https://doi.org/10.1016/j.immuni.2010.01.012
Sasawatari, Shigemi ; Yoshizaki, Mariko ; Taya, Choji ; Tazawa, Aya ; Furuyama-Tanaka, Kaori ; Yonekawa, Hiromichi ; Dohi, Taeko ; Makrigiannis, Andrew P. ; Sasazuki, Takehiko ; Inaba, Kayo ; Toyama-Sorimachi, Noriko. / The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking. :: Immunity. 2010 ; 巻 32, 番号 2. pp. 200-213.
@article{cf7f69a94ccb4f14944829145efbcf9f,
title = "The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking",
abstract = "Neutrophils rapidly undergo polarization and directional movement to infiltrate the sites of infection and inflammation. Here, we show that an inhibitory MHC I receptor, Ly49Q, was crucial for the swift polarization of and tissue infiltration by neutrophils. During the steady state, Ly49Q inhibited neutrophil adhesion by preventing focal-complex formation, likely by inhibiting Src and PI3 kinases. However, in the presence of inflammatory stimuli, Ly49Q mediated rapid neutrophil polarization and tissue infiltration in an ITIM-domain-dependent manner. These opposite functions appeared to be mediated by distinct use of effector phosphatase SHP-1 and SHP-2. Ly49Q-dependent polarization and migration were affected by Ly49Q regulation of membrane raft functions. We propose that Ly49Q is pivotal in switching neutrophils to their polarized morphology and rapid migration upon inflammation, through its spatiotemporal regulation of membrane rafts and raft-associated signaling molecules.",
author = "Shigemi Sasawatari and Mariko Yoshizaki and Choji Taya and Aya Tazawa and Kaori Furuyama-Tanaka and Hiromichi Yonekawa and Taeko Dohi and Makrigiannis, {Andrew P.} and Takehiko Sasazuki and Kayo Inaba and Noriko Toyama-Sorimachi",
year = "2010",
month = "2",
day = "26",
doi = "10.1016/j.immuni.2010.01.012",
language = "English",
volume = "32",
pages = "200--213",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "2",

}

TY - JOUR

T1 - The Ly49Q Receptor Plays a Crucial Role in Neutrophil Polarization and Migration by Regulating Raft Trafficking

AU - Sasawatari, Shigemi

AU - Yoshizaki, Mariko

AU - Taya, Choji

AU - Tazawa, Aya

AU - Furuyama-Tanaka, Kaori

AU - Yonekawa, Hiromichi

AU - Dohi, Taeko

AU - Makrigiannis, Andrew P.

AU - Sasazuki, Takehiko

AU - Inaba, Kayo

AU - Toyama-Sorimachi, Noriko

PY - 2010/2/26

Y1 - 2010/2/26

N2 - Neutrophils rapidly undergo polarization and directional movement to infiltrate the sites of infection and inflammation. Here, we show that an inhibitory MHC I receptor, Ly49Q, was crucial for the swift polarization of and tissue infiltration by neutrophils. During the steady state, Ly49Q inhibited neutrophil adhesion by preventing focal-complex formation, likely by inhibiting Src and PI3 kinases. However, in the presence of inflammatory stimuli, Ly49Q mediated rapid neutrophil polarization and tissue infiltration in an ITIM-domain-dependent manner. These opposite functions appeared to be mediated by distinct use of effector phosphatase SHP-1 and SHP-2. Ly49Q-dependent polarization and migration were affected by Ly49Q regulation of membrane raft functions. We propose that Ly49Q is pivotal in switching neutrophils to their polarized morphology and rapid migration upon inflammation, through its spatiotemporal regulation of membrane rafts and raft-associated signaling molecules.

AB - Neutrophils rapidly undergo polarization and directional movement to infiltrate the sites of infection and inflammation. Here, we show that an inhibitory MHC I receptor, Ly49Q, was crucial for the swift polarization of and tissue infiltration by neutrophils. During the steady state, Ly49Q inhibited neutrophil adhesion by preventing focal-complex formation, likely by inhibiting Src and PI3 kinases. However, in the presence of inflammatory stimuli, Ly49Q mediated rapid neutrophil polarization and tissue infiltration in an ITIM-domain-dependent manner. These opposite functions appeared to be mediated by distinct use of effector phosphatase SHP-1 and SHP-2. Ly49Q-dependent polarization and migration were affected by Ly49Q regulation of membrane raft functions. We propose that Ly49Q is pivotal in switching neutrophils to their polarized morphology and rapid migration upon inflammation, through its spatiotemporal regulation of membrane rafts and raft-associated signaling molecules.

UR - http://www.scopus.com/inward/record.url?scp=76949101548&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=76949101548&partnerID=8YFLogxK

U2 - 10.1016/j.immuni.2010.01.012

DO - 10.1016/j.immuni.2010.01.012

M3 - Article

C2 - 20153219

AN - SCOPUS:76949101548

VL - 32

SP - 200

EP - 213

JO - Immunity

JF - Immunity

SN - 1074-7613

IS - 2

ER -