TY - JOUR
T1 - The MMP activity in developing rat molar roots and incisors demonstrated by in situ zymography
AU - Sakuraba, Ichiro
AU - Hatakeyama, Junko
AU - Hatakeyama, Yuji
AU - Takahashi, Ichiro
AU - Mayanagi, Hideaki
AU - Sasano, Yasuyuki
N1 - Funding Information:
Acknowledgements This work was supported in part by grants-in-aid (15390550, 16500285, 17659573, 17791296, 17791508) from the Ministry of Education, Science, Sports and Culture of Japan.We wish to thank Mr. Masami Eguchi and Mr. Yasuto Mi-kami, Division of Craniofacial Development and Regeneration, Tohoku University Graduate School of Dentistry, for their excellent assistance in this study.
PY - 2006/1
Y1 - 2006/1
N2 - Matrix metalloproteinases (MMPs) have been expressed during root development and periodontal tissue formation, whereas it is not known if these MMP molecules are enzymatically active to degrade the extracellular matrices (ECMs). The present study was designed to investigate the gelatinolytic and collagenolytic activity in rat molar root and incisor development. Three-week old rat mandibles were frozen and cut without fixation or decalcification and processed for in situ zymography using substrates gelatin and collagen. The enzymatic activity was assessed according to the intensity of fluorescence due to the lysis of the substrates. Odontoblasts, predentin, cementum, bone and the enamel matrix showed the high activity. The present study demonstrated MMP activity in calcified tissues using in situ zymography for the first time and the possible involvement of the MMP activity in molar root and incisor development and periodontal tissue formation.
AB - Matrix metalloproteinases (MMPs) have been expressed during root development and periodontal tissue formation, whereas it is not known if these MMP molecules are enzymatically active to degrade the extracellular matrices (ECMs). The present study was designed to investigate the gelatinolytic and collagenolytic activity in rat molar root and incisor development. Three-week old rat mandibles were frozen and cut without fixation or decalcification and processed for in situ zymography using substrates gelatin and collagen. The enzymatic activity was assessed according to the intensity of fluorescence due to the lysis of the substrates. Odontoblasts, predentin, cementum, bone and the enamel matrix showed the high activity. The present study demonstrated MMP activity in calcified tissues using in situ zymography for the first time and the possible involvement of the MMP activity in molar root and incisor development and periodontal tissue formation.
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U2 - 10.1007/s10735-006-9037-6
DO - 10.1007/s10735-006-9037-6
M3 - Article
C2 - 16830066
AN - SCOPUS:33748850395
SN - 1567-2379
VL - 37
SP - 87
EP - 93
JO - Journal of Molecular Histology
JF - Journal of Molecular Histology
IS - 1-2
ER -