TY - JOUR
T1 - The novel heme-dependent inducible protein, SRRD regulates heme biosynthesis and circadian rhythms
AU - Adachi, Yuka
AU - Umeda, Mana
AU - Kawazoe, Asako
AU - Sato, Tetsuya
AU - Ohkawa, Yasuyuki
AU - Kitajima, Sakihito
AU - Izawa, Shingo
AU - Sagami, Ikuko
AU - Taketani, Shigeru
N1 - Funding Information:
The authors thank Dr. Osamu Nakajima and Dr. Ken Itoh for the kind gifts of pCAG-ALAS1 and pHO-1 enhancer luc, respectively, Dr. Kazushige Hamada for his valuable advice, and Ms. Machiko Sasai, Chihiro Hori and Afeng Mu for their expert technical assistance. This study was supported in part by grants from the Ministry of Health, Labor and Welfare of Japan ( H29-Nanchi-R-021 ). Appendix A
PY - 2017/10/1
Y1 - 2017/10/1
N2 - Heme plays a role in the regulation of the expression of genes related to circadian rhythms and heme metabolism. In order to identify new heme-regulated proteins, an RNA sequence analysis using mouse NIH3T3 cells treated without or with 5-aminolevulinic acid (ALA) was performed. Among the changes observed in the levels of various mRNAs including heme oxygenase-1 (HO-1) and ALA synthase-1 (ALAS1), a mouse homologue of the plant circadian-regulating protein SRR1, SRR1 domain containing (SRRD) was induced by the ALA treatment. The expression of SRRD was dependent on heme biosynthesis, and increased the production of heme. SRRD was expressed under circadian rhythms, and influenced the expression of clock genes including PER2, BMAL1, and CLOCK. The knockout of SRRD arrested the growth of cells, indicating that SRRD plays roles in heme-regulated circadian rhythms and cell proliferation.
AB - Heme plays a role in the regulation of the expression of genes related to circadian rhythms and heme metabolism. In order to identify new heme-regulated proteins, an RNA sequence analysis using mouse NIH3T3 cells treated without or with 5-aminolevulinic acid (ALA) was performed. Among the changes observed in the levels of various mRNAs including heme oxygenase-1 (HO-1) and ALA synthase-1 (ALAS1), a mouse homologue of the plant circadian-regulating protein SRR1, SRR1 domain containing (SRRD) was induced by the ALA treatment. The expression of SRRD was dependent on heme biosynthesis, and increased the production of heme. SRRD was expressed under circadian rhythms, and influenced the expression of clock genes including PER2, BMAL1, and CLOCK. The knockout of SRRD arrested the growth of cells, indicating that SRRD plays roles in heme-regulated circadian rhythms and cell proliferation.
UR - http://www.scopus.com/inward/record.url?scp=85027986561&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85027986561&partnerID=8YFLogxK
U2 - 10.1016/j.abb.2017.08.006
DO - 10.1016/j.abb.2017.08.006
M3 - Article
C2 - 28802827
AN - SCOPUS:85027986561
SN - 0003-9861
VL - 631
SP - 19
EP - 29
JO - Archives of Biochemistry and Biophysics
JF - Archives of Biochemistry and Biophysics
ER -