The peroxin Pex14p: cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis

Nobuhiro Shimizu, Ryota Itoh, Yoko Hirono, Hidenori Otera, Kamran Ghaedi, Keita Tateishi, Shigehiko Tamura, Kanji Okumoto, Tomoyuki Harano, Satoru Mukai, Yukio Fujiki

研究成果: ジャーナルへの寄稿記事

128 引用 (Scopus)

抄録

Rat cDNA encoding a 376-amino acid peroxin was isolated by functional complementation of a peroxisome-deficient Chinese hamster ovary cell mutant, ZP110, of complementation group 14 (CG14). The primary sequence showed 28 and 24% amino acid identity with the yeast Pex14p from Hansenula polymorpha and Saccharomyces cerevisiae, respectively; therefore, we termed this cDNA rat PEX14 (RnPEX14). Human and Chinese hamster Pex14p showed 96 and 94% identity to rat Pex14p, except that both Pex14p comprised 377 amino acids. Pex14p was characterized as an integral membrane protein of peroxisomes, exposing its N- and C-terminal parts to the cytosol. Pex14p interacts with both Pex5p and Pex7p, the receptors for peroxisome targeting signal type 1 (PTS1) and PTS2, respectively, together with the receptors' cargoes, PTS1 and PTS2 proteins. Mutation in PEX14 from ZP161, the same CG as ZP110, was determined by reverse transcription-PCR as follows. A 133-base pair deletion at nucleotide residues 37-169 in one allele created a termination codon at 40-42; in addition to this mutation, 103 base pairs were deleted at positions 385-487, resulting in the second termination immediately downstream the second deletion site in the other allele. Neither of these two mutant forms of Pex14p restored peroxisome biogenesis in ZP110 and ZP161, thereby demonstrating PEX14 to be responsible for peroxisome deficiency in CG14.

元の言語英語
ページ(範囲)12593-12604
ページ数12
ジャーナルJournal of Biological Chemistry
274
発行部数18
DOI
出版物ステータス出版済み - 4 30 1999

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Functional analysis
Peroxisomes
Cloning
Cricetulus
Rats
Organism Cloning
Ovary
Complementary DNA
Cells
Amino Acids
Yeast
Base Pairing
Alleles
Transcription
Mutation
Pichia
Terminator Codon
Membrane Proteins
Nucleotides
Cytosol

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

これを引用

The peroxin Pex14p : cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis. / Shimizu, Nobuhiro; Itoh, Ryota; Hirono, Yoko; Otera, Hidenori; Ghaedi, Kamran; Tateishi, Keita; Tamura, Shigehiko; Okumoto, Kanji; Harano, Tomoyuki; Mukai, Satoru; Fujiki, Yukio.

:: Journal of Biological Chemistry, 巻 274, 番号 18, 30.04.1999, p. 12593-12604.

研究成果: ジャーナルへの寄稿記事

Shimizu, Nobuhiro ; Itoh, Ryota ; Hirono, Yoko ; Otera, Hidenori ; Ghaedi, Kamran ; Tateishi, Keita ; Tamura, Shigehiko ; Okumoto, Kanji ; Harano, Tomoyuki ; Mukai, Satoru ; Fujiki, Yukio. / The peroxin Pex14p : cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis. :: Journal of Biological Chemistry. 1999 ; 巻 274, 番号 18. pp. 12593-12604.
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title = "The peroxin Pex14p: cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis",
abstract = "Rat cDNA encoding a 376-amino acid peroxin was isolated by functional complementation of a peroxisome-deficient Chinese hamster ovary cell mutant, ZP110, of complementation group 14 (CG14). The primary sequence showed 28 and 24{\%} amino acid identity with the yeast Pex14p from Hansenula polymorpha and Saccharomyces cerevisiae, respectively; therefore, we termed this cDNA rat PEX14 (RnPEX14). Human and Chinese hamster Pex14p showed 96 and 94{\%} identity to rat Pex14p, except that both Pex14p comprised 377 amino acids. Pex14p was characterized as an integral membrane protein of peroxisomes, exposing its N- and C-terminal parts to the cytosol. Pex14p interacts with both Pex5p and Pex7p, the receptors for peroxisome targeting signal type 1 (PTS1) and PTS2, respectively, together with the receptors' cargoes, PTS1 and PTS2 proteins. Mutation in PEX14 from ZP161, the same CG as ZP110, was determined by reverse transcription-PCR as follows. A 133-base pair deletion at nucleotide residues 37-169 in one allele created a termination codon at 40-42; in addition to this mutation, 103 base pairs were deleted at positions 385-487, resulting in the second termination immediately downstream the second deletion site in the other allele. Neither of these two mutant forms of Pex14p restored peroxisome biogenesis in ZP110 and ZP161, thereby demonstrating PEX14 to be responsible for peroxisome deficiency in CG14.",
author = "Nobuhiro Shimizu and Ryota Itoh and Yoko Hirono and Hidenori Otera and Kamran Ghaedi and Keita Tateishi and Shigehiko Tamura and Kanji Okumoto and Tomoyuki Harano and Satoru Mukai and Yukio Fujiki",
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T1 - The peroxin Pex14p

T2 - cDNA cloning by functional complementation on a Chinese hamster ovary cell mutant, characterization, and functional analysis

AU - Shimizu, Nobuhiro

AU - Itoh, Ryota

AU - Hirono, Yoko

AU - Otera, Hidenori

AU - Ghaedi, Kamran

AU - Tateishi, Keita

AU - Tamura, Shigehiko

AU - Okumoto, Kanji

AU - Harano, Tomoyuki

AU - Mukai, Satoru

AU - Fujiki, Yukio

PY - 1999/4/30

Y1 - 1999/4/30

N2 - Rat cDNA encoding a 376-amino acid peroxin was isolated by functional complementation of a peroxisome-deficient Chinese hamster ovary cell mutant, ZP110, of complementation group 14 (CG14). The primary sequence showed 28 and 24% amino acid identity with the yeast Pex14p from Hansenula polymorpha and Saccharomyces cerevisiae, respectively; therefore, we termed this cDNA rat PEX14 (RnPEX14). Human and Chinese hamster Pex14p showed 96 and 94% identity to rat Pex14p, except that both Pex14p comprised 377 amino acids. Pex14p was characterized as an integral membrane protein of peroxisomes, exposing its N- and C-terminal parts to the cytosol. Pex14p interacts with both Pex5p and Pex7p, the receptors for peroxisome targeting signal type 1 (PTS1) and PTS2, respectively, together with the receptors' cargoes, PTS1 and PTS2 proteins. Mutation in PEX14 from ZP161, the same CG as ZP110, was determined by reverse transcription-PCR as follows. A 133-base pair deletion at nucleotide residues 37-169 in one allele created a termination codon at 40-42; in addition to this mutation, 103 base pairs were deleted at positions 385-487, resulting in the second termination immediately downstream the second deletion site in the other allele. Neither of these two mutant forms of Pex14p restored peroxisome biogenesis in ZP110 and ZP161, thereby demonstrating PEX14 to be responsible for peroxisome deficiency in CG14.

AB - Rat cDNA encoding a 376-amino acid peroxin was isolated by functional complementation of a peroxisome-deficient Chinese hamster ovary cell mutant, ZP110, of complementation group 14 (CG14). The primary sequence showed 28 and 24% amino acid identity with the yeast Pex14p from Hansenula polymorpha and Saccharomyces cerevisiae, respectively; therefore, we termed this cDNA rat PEX14 (RnPEX14). Human and Chinese hamster Pex14p showed 96 and 94% identity to rat Pex14p, except that both Pex14p comprised 377 amino acids. Pex14p was characterized as an integral membrane protein of peroxisomes, exposing its N- and C-terminal parts to the cytosol. Pex14p interacts with both Pex5p and Pex7p, the receptors for peroxisome targeting signal type 1 (PTS1) and PTS2, respectively, together with the receptors' cargoes, PTS1 and PTS2 proteins. Mutation in PEX14 from ZP161, the same CG as ZP110, was determined by reverse transcription-PCR as follows. A 133-base pair deletion at nucleotide residues 37-169 in one allele created a termination codon at 40-42; in addition to this mutation, 103 base pairs were deleted at positions 385-487, resulting in the second termination immediately downstream the second deletion site in the other allele. Neither of these two mutant forms of Pex14p restored peroxisome biogenesis in ZP110 and ZP161, thereby demonstrating PEX14 to be responsible for peroxisome deficiency in CG14.

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